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Gene Expression Data
Assay Details
Assay
Reference: J:99569 Barrow KM, et al., Embryonic expression of murine 5T4 oncofoetal antigen is associated with morphogenetic events at implantation and in developing epithelia. Dev Dyn. 2005 Aug;233(4):1535-45
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3603423
Gene symbol: Tpbg
Gene name: trophoblast glycoprotein
Antibody: anti-5T4
Detection system: Secondary antibody coupled to fluorescein
Assay notes: Fixative was 1% paraformaldehyde.
Results
Specimen Table 1: embryonic day 0.5 (more )
Note: Fertilized oocyte.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Absent

Specimen Table 1: embryonic day 1.0 (more )
Note: 2-cell embryo.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Absent

Specimen Table 1: embryonic day 2.0 (more )
Note: 4-cell embryo.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Absent

Specimen Table 1: embryonic day 2.5 (more )
Note: 8-cell embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent

Specimen Table 1: embryonic day 3.0 (more )
Note: Morula.
Structure Level Pattern Image Note
TS3: compacted morula Absent

Specimen Table 1: embryonic day 3.5 (more )
Note: E3.5 blastocyst.
Structure Level Pattern Image Note
TS4: blastocyst Absent

Specimen Table 1: embryonic day 4.0 (more )
Note: Hatched blastocyst.
Structure Level Pattern Image Note
TS5: blastocyst Present About half the blastocysts were positive.

Specimen 1Bi: embryonic day 0.5 (more )
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Absent 1B(i)

Specimen 1Bii: embryonic day 1.0 (more )
Note: 2-cell embryo.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Absent 1B(ii)

Specimen 1Biii: embryonic day 2.5 (more )
Note: 8-cell embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 1B(iii)

Specimen 1Biv: embryonic day 3.5 (more )
Note: E3.5 blastocyst.
Structure Level Pattern Image Note
TS4: blastocyst Absent 1B(iv)

Specimen 1C: embryonic day 4.0 (more )
Note: Hatched blastocyst.
Structure Level Pattern Image Note
TS5: blastocyst Present 1C(i) , 1C(iii)

Specimen 1D: embryonic day 4.0 (more )
Note: Hatched blastocyst. Double labeled: green - Tpbg; red - Pou5f1 (OCT-4; assay MGI:3603429).
Structure Level Pattern Image Note
TS5: trophectoderm Present 1D right , 1D center , 1D left
TS5: inner cell mass Absent 1D center , 1D left , 1D right

Specimen 6A: embryonic day 15.5 (more )
Note: Double labeled: green - Tpbg; red - Krt14 (assay MGI:3603463).
Structure Level Pattern Image Note
TS23: vibrissa Present 6A 5T4 , 6A merge

Specimen 6B: embryonic day 15.5 (more )
Note: Double labeled: green - Tpbg; red - Krt14 (assay MGI:3603463).
Structure Level Pattern Image Note
TS23: epidermis Present 6B merge , 6B 5T4


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory