Mecp2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:97538 Pelka GJ, et al., Distinct expression profiles of Mecp2 transcripts with different lengths of 3'UTR in the brain and visceral organs during mouse development. Genomics. 2005 Apr;85(4):441-52
Assay type:	RT-PCR
MGI Accession ID:	MGI:3603132
Gene symbol:	Mecp2
Gene name:	methyl CpG binding protein 2

Probe:	Mecp2-pE, Mesp2-pF
Assay notes:	Results were obtained using real-time PCR. They are depicted graphically in Fig. 3E.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
16.5dpc	E16.5 (a)	TS24: cerebral cortex	Present (b)	500 µg; total RNA	129X1/SvJ		Not Specified	occipital cortex
2wk	P w 2	TS28: occipital cortex	Present (c)	500 µg; total RNA	129X1/SvJ		Not Specified
12wk	P w 12	TS28: occipital cortex	Weak (d)	500 µg; total RNA	129X1/SvJ		Not Specified
20wk	P w 20	TS28: occipital cortex	Weak (e)	500 µg; total RNA	129X1/SvJ		Not Specified
60wk	P w 60	TS28: occipital cortex	Weak (f)	500 µg; total RNA	129X1/SvJ		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression was measured at approximately 75 arbitrary units.
(c) Expression was measured at approximately 50 arbitrary units.
(d) Expression was measured at approximately 20 arbitrary units.
(e) Expression was measured at approximately 40 arbitrary units.
(f) Expression was measured at approximately 45 arbitrary units.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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