Antibody:	anti-Pem
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Absent		2A

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Present		2B	Expression is present in 12% of compacted morula nuclei. Practically speaking, this is generally one positive cell per morula, although one observed morula contained seven strongly positive cells.

Structure	Level	Pattern	Image	Note
TS5: trophectoderm	Present		2C	Expression is present in 25% of the trophectoderm cells.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		2D	Expression is present in 15% of the inner cell mass cells.

Structure	Level	Pattern	Image	Note
TS7: ectoplacental cone	Strong		3A
TS7: parietal endoderm	Strong		3A
TS7: extraembryonic visceral endoderm	Strong	Regionally restricted	3A	Expression is present in the visceral endoderm that surrounds the embryo.
TS7: embryo	Absent		3A	There is no expression in the embryonic ectoderm.
TS28: decidua	Absent		3A

Structure	Level	Pattern	Image	Note
TS9: extraembryonic visceral endoderm	Present		3B	Expression is present in the distal visceral endoderm.
TS9: parietal endoderm	Present		3B

Structure	Level	Pattern	Image	Note
TS10: extraembryonic visceral endoderm	Present	Regionally restricted	3C	Expression levels are high in the proximal visceral endoderm, whereas expression in the distal visceral endoerm is reduced from what was seen earlier in development.
TS10: parietal endoderm	Present		3C	Expression levels are reduced from what was seen earlier in development.
TS10: ectoplacental cone	Strong		3C

Structure	Level	Pattern	Image	Note
TS10: extraembryonic visceral endoderm	Present	Regionally restricted	3D	Expression levels are high in the proximal visceral endoderm, whereas expression in the distal visceral endoerm is reduced from what was seen earlier in development.
TS10: parietal endoderm	Present		3D	Expression levels are reduced from what was seen earlier in development.
TS10: ectoplacental cone	Strong		3D

Structure	Level	Pattern	Image	Note
TS11: extraembryonic visceral endoderm	Present	Regionally restricted	3E	Expression levels are high in the proximal visceral endoderm, while there is no detectable expression in the distal visceral endoderm.
TS11: parietal endoderm	Absent		3E
TS11: trophectoderm	Present	Regionally restricted	3E	Expression is found in some, but not all, secondary giant trophoblast cells.
TS11: chorion	Strong		3E
TS11: amnion	Absent		3E
TS11: embryo ectoderm	Absent		3E
TS11: embryo mesenchyme	Absent		3E

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Present	Regionally restricted		The pattern of expression seen in extraembryonic tissues remains unchanged from what was seen at E7.5.