Antibody:	anti-tenascin
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present	Regionally restricted	2D	Expression is surrounding the airways.

Structure	Level	Pattern	Image	Note
TS25: trachea smooth muscle	Present		3B
TS25: trachea epithelium	Present	Regionally restricted	3B	Expression is at the epithelial/mesenchymal interface.
TS25: trachea mesenchyme	Present	Regionally restricted	3B	Expression is at the epithelial/mesenchymal interface.

Structure	Level	Pattern	Image	Note
TS25: lung	Present	Regionally restricted	3D	Expression is in the nascent alveolar septa.

Structure	Level	Pattern	Image	Note
TS20: trachea	Present	Regionally restricted	4C	Expression is at the epithelial/mesenchymal interface.
TS20: esophagus	Present	Regionally restricted	4C	Expression is in the matrix surrounding smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS20: arterial system	Present	Regionally restricted	4F	Expression is encircling small- and medium-sized arteries.
TS20: gut	Absent		4F

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Present	Non-Uniform	4I

Structure	Level	Pattern	Image	Note
TS20: intraembryonic coelom	Strong	Regionally restricted		Expression is in the diaphragm.
TS20: cartilage	Strong
TS20: limb	Strong
TS20: skeleton	Strong
TS20: thymus primordium	Strong
TS20: tongue	Strong
TS20: pancreas	Absent
TS20: thyroid gland	Absent

Structure	Level	Pattern	Image	Note
TS25: esophagus	Moderate	Regionally restricted	5B	Expression is in smooth muscle cells.
TS25: esophagus epithelium	Present	Regionally restricted	5B	Expression is at the epithelial/mesenchymal interface.
TS25: esophagus mesenchyme	Present	Regionally restricted	5B	Expression is at the epithelial/mesenchymal interface.
TS25: blood vessel	Present	Regionally restricted	5B	Expression is in vascular smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS25: esophagus	Moderate	Regionally restricted	5D	Expression is in smooth muscle cells.
TS25: esophagus epithelium	Present	Regionally restricted	5D	Expression is at the epithelial/mesenchymal interface.
TS25: esophagus mesenchyme	Present	Regionally restricted	5D	Expression is at the epithelial/mesenchymal interface.

Structure	Level	Pattern	Image	Note
TS25: tongue muscle	Moderate		6B

Structure	Level	Pattern	Image	Note
TS25: vibrissa	Present	Regionally restricted	7B	Expression is at the dermo-epithelial junction completely around nascent follicles; the hair germs and adjacent dermal papilla of the more mature vibrissae express little or no protein.

Structure	Level	Pattern	Image	Note
TS25: vibrissa	Present	Regionally restricted	7D	Expression is at the dermo-epithelial junction completely around nascent follicles; the hair germs and adjacent dermal papilla of the more mature vibrissae express little or no protein.

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Weak	Regionally restricted	8B	Expression is in the mesenchymal-epithelial interfcae.

Structure	Level	Pattern	Image	Note
TS25: limb	Present	Regionally restricted		Expression in muscle has declined but is strong in tendons and myotendinous junctions.
TS25: cartilage	Strong
TS25: skeleton	Strong
TS25: thymus	Strong
TS25: pancreas	Absent
TS25: thyroid gland	Absent