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Gene Expression Data
Assay Details
Assay
Reference: J:98482 Bouchard M, et al., Identification of Pax2-regulated genes by expression profiling of the mid-hindbrain organizer region. Development. 2005 Jun;132(11):2633-43
Assay type: RNA in situ
MGI Accession ID: MGI:3586674
Gene symbol: Pou3f3
Gene name: POU domain, class 3, transcription factor 3
Probe: Brn1 cDNA1
Probe preparation: labelled with digoxigenin RNA
Results
Specimen 2E: embryonic day 8.5 (more )
Note: Embryo analyzed had 8-10 somites.
Structure Level Pattern Image Note
TS13: future midbrain Present 2E
TS13: future hindbrain Present Regionally restricted 2E Expression was detected in the anterior hindbrain.
Specimen 2F: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS14: future hindbrain Present 2F
TS14: prosencephalon Present 2F
TS14: future midbrain Present Uniform 2F Expression was detected throughout the entire midbrain.
Specimen 3C: embryonic day 8.0-8.5 (more )
Note: Embryo analyzed had 7-8 somites. This embryo was either Pax2+/+ or Pax2+/-.
Structure Level Pattern Image Note
TS12: future hindbrain Present 3C
TS12: future midbrain Present 3C
Specimen 3D: embryonic day 8.0-8.5; Pax2tm1Mbu/Pax2tm1Mbu (more )
Note: Embryo analyzed had 7-8 somites.
Structure Level Pattern Image Note
TS12: future hindbrain Absent 3D
TS12: future midbrain Absent 3D
Specimen S3A 5s: embryonic day 8.0 (more )
Note: Embryo analyzed had 5 somites.
Structure Level Pattern Image Note
TS12: future hindbrain Present S3A 5s Initiation of expression during early somitogenesis.
TS12: future midbrain Present S3A 5s Initiation of expression during early somitogenesis.
Specimen S3A 6s: embryonic day 8.0 (more )
Note: Embryo analyzed had 6 somites.
Structure Level Pattern Image Note
TS12: future hindbrain Present S3A 6s Initiation of expression during early somitogenesis.
TS12: future midbrain Present S3A 6s Initiation of expression during early somitogenesis.
Specimen S3A 7s: embryonic day 8.0 (more )
Note: Embryo analyzed had 7 somites.
Structure Level Pattern Image Note
TS12: future hindbrain Present S3A 7s Initiation of expression during early somitogenesis.
TS12: future midbrain Present S3A 7s Initiation of expression during early somitogenesis.
Specimen S3B Pax5 +/-: embryonic day 9.5; Pax5tm1Mbu/Pax5+ (more )
Structure Level Pattern Image Note
TS14: future hindbrain Present S3B Pax5 +/-
TS14: future midbrain Present S3B Pax5 +/-
Specimen S3B Pax5 -/-: embryonic day 9.5; Pax5tm1Mbu/Pax5tm1Mbu (more )
Structure Level Pattern Image Note
TS15: future hindbrain Present S3B Pax5 -/- As in wild type, expression was detected in the mid-hindbrain region.
TS15: future midbrain Present S3B Pax5 -/- As in wild type, expression was detected in the mid-hindbrain region.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory