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Gene Expression Data
Assay Details
Assay
Reference: J:98500 Meech R, et al., The homeobox transcription factor Barx2 regulates chondrogenesis during limb development. Development. 2005 May;132(9):2135-46
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3586389
Gene symbol: Col2a1
Gene name: collagen, type II, alpha 1
Antibody: Abcam Clone 5B2.5
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2A, D: embryonic day 11.0 (more )
Note: Double labeled: green - Col2a1; red - Barx2 (assay MGI:3586388). D is a higher magnification of a portion of A.
Structure Level Pattern Image Note
TS18: limb mesenchyme Present Regionally restricted 2A , 2D Expression detected in the nuclei of the condensing pre-chondrogenic mesenchyme.

Specimen 2B, E: embryonic day 12.5 (more )
Note: Double labeled: green - Col2a1; red - Barx2 (assay MGI:3586388). E is a higher magnification of a portion of B.
Structure Level Pattern Image Note
TS20: limb mesenchyme Trace Regionally restricted 2B , 2E Expression detected in joint interzones.
TS20: limb digit Present Regionally restricted 2E , 2B Expression detected in the digital rays.

Specimen 2C: postnatal newborn (more )
Note: Double labeled: green - Col2a1; red - Barx2 (assay MGI:3586388).
Structure Level Pattern Image Note
TS27: autopod joint Present Regionally restricted 2C Expression detected in the articular cartilage of carpal joints.

Specimen 2F: postnatal newborn (more )
Note: Double labeled: green - Col2a1; red - Barx2 (assay MGI:3586388).
Structure Level Pattern Image Note
TS27: autopod joint Present Regionally restricted 2F Expression detected in the articular cartilage of phalangeal joints.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory