Probe:	IMAGE clone 420533
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	This data is from Supplemental Table 5, Gene column, row: 284344 Naked cuticle 1 homolog . After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde prior to processing.

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present	Regionally restricted	S11_Nkd1 E14 , Submitted Image - ab3e14 , Submitted Image - ab3e14b	Expression was detected in a subset of cells in the outer neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS24: retina	Present	Uniform	Submitted Image - ab3e16 , S11_Nkd1 E16	Expression was panretinal.

Structure	Level	Pattern	Image	Note
TS26: retina outer nuclear layer	Weak	Regionally restricted	Submitted Image - AB3e18.5 , Submitted Image - ab3e18 , S11_Nkd1 E18 , Submitted Image - ab3e18b	Expression was detected in a subset of cells in the outer neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS27: retina outer nuclear layer	Weak	Regionally restricted	S11_Nkd1 P0 , Submitted Image - AB3p0.5 , Submitted Image - ab3p0b	Expression was detected in a subset of cells in the outer neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS27: retina outer nuclear layer	Present	Regionally restricted	S11_Nkd1 P2 , Submitted Image - ab3p2b , Submitted Image - AB3p3.5	Expression was detected in the vitreal outer neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present	Regionally restricted	Submitted Image - ab3p4 , S11_Nkd1 P4	Expression was detected in the vitreal outer neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	Submitted Image - ab3p6 , Submitted Image - ab3p6b , S11_Nkd1 P6 , Submitted Image - AB3p6.5	Expression was detected in the Muller glia.

Structure	Level	Pattern	Image	Note
TS28: retina	Weak	Regionally restricted	Submitted Image - ab3p10 , Submitted Image - AB3p10.5 , Submitted Image - ab3p8	Expression was detected in the Muller glia.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	Submitted Image - ab3ade , Submitted Image - AB3adult , S11_Nkd1 Adult	Expression was detected in the Muller glia.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present
TS28: cerebellum	Present
TS28: hippocampus	Present
TS28: blood vessel	Present

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Present			Expression was detected in the central nervous system in a complex pattern.
TS24: head	Present	Regionally restricted		Expression was detected in the face in a complex pattern.