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Gene Expression Data
Assay Details
Assay
Reference: J:98712 Yamaguchi S, et al., Nanog expression in mouse germ cell development. Gene Expr Patterns. 2005 Jun;5(5):639-646
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3584425
Gene symbol: Nanog
Gene name: Nanog homeobox
Antibody: anti-Nanog
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Results are summarized in Table 1.
Results
Specimen 1A-E7.5: embryonic day 7.5 (more )
Note: Double labeled: red - Nanog; green - Dppa3 (PGC7; assay MGI:3584539).
Structure Level Pattern Image Note
TS11: primordial germ cell Absent 1A E7.5
Specimen 1A-E7.75: embryonic day 7.75 (more )
Note: Double labeled: red - Nanog; green - Dppa3 (PGC7; assay MGI:3584539).
Structure Level Pattern Image Note
TS12: primordial germ cell Present Single cells 1A E7.75 The positive cells are at the proximal region of the allantois.
Specimen 1B-E7.75: embryonic day 7.75 (more )
Note: Double labeled: red - Nanog; green - Dppa3 (PGC7; assay MGI:3584539).
Structure Level Pattern Image Note
TS12: primordial germ cell Present Single cells 1B E7.75 NANOG , 1B E7.75 MERGE
Specimen 1B-E8.5: embryonic day 8.5 (more )
Note: Double labeled: red - Nanog; green - Dppa3 (PGC7; assay MGI:3584539).
Structure Level Pattern Image Note
TS13: primordial germ cell Present Single cells 1B E8.5 NANOG , 1B E8.5 MERGE
Specimen 2A: embryonic day 9.5 (more )
Note: Double labeled: red - Nanog; green - Dppa3 (PGC7; assay MGI:3584539).
Structure Level Pattern Image Note
TS15: primordial germ cell Present Single cells 2A NANOG , 2A
Specimen 2B: embryonic day 10.5 (more )
Note: Double labeled: red - Nanog; green - Dppa3 (PGC7; assay MGI:3584539).
Structure Level Pattern Image Note
TS17: primordial germ cell Present Single cells 2B , 2B NANOG
Specimen 3A: embryonic day 11.5 (more )
Note: Double labeled: red - Nanog; green - Dppa3 (PGC7; assay MGI:3584539).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present Single cells 3A NANOG , 3A
Specimen 3B: embryonic day 12.5 (more )
Note: Double labeled: red - Nanog; green - Dppa3 (PGC7; assay MGI:3584539).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present Single cells 3B NANOG , 3B
Specimen 4A-E13.5: embryonic day 13.5 (more )
Note: Double labeled: red - Nanog; green - Dppa3 (PGC7; assay MGI:3584539).
Structure Level Pattern Image Note
TS21: germ cell of ovary Present Single cells 4A E13.5 , 4A E13.5 NANOG
Specimen 4A-E14.5: embryonic day 14.5 (more )
Note: Double labeled: red - Nanog; green - Sycp3 (assay MGI:3584540).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present Single cells 4A E14.5 , 4A E14.5 NANOG Fewer cells stained positive compared to earlier ages.
Specimen 4A-E15.5: embryonic day 15.5 (more )
Note: Double labeled: red - Nanog; green - Sycp3 (assay MGI:3584540).
Structure Level Pattern Image Note
TS23: germ cell of ovary Absent 4A E15.5 NANOG , 4A E15.5
Specimen 4B-E13.5: embryonic day 13.5 (more )
Note: Double labeled: red - Nanog; green - Dppa3 (PGC7; assay MGI:3584539).
Structure Level Pattern Image Note
TS21: germ cell of testis Present Single cells 4B E13.5 NANOG , 4B E13.5
Specimen 4B-E14.5: embryonic day 14.5 (more )
Note: Double labeled: red - Nanog; green - Dppa3 (PGC7; assay MGI:3584539).
Structure Level Pattern Image Note
TS22: germ cell of testis Present Single cells 4B E14.5 , 4B E14.5 NANOG Fewer cells stained positive compared to earlier ages.
Specimen 4B-E15.5: embryonic day 15.5 (more )
Note: Double labeled: red - Nanog; green - Dppa3 (PGC7; assay MGI:3584539).
Structure Level Pattern Image Note
TS23: germ cell of testis Present Single cells 4B E15.5 , 4B E15.5 NANOG Only a few cells stained positive.
Specimen 4B-E16.5: embryonic day 16.5 (more )
Note: Double labeled: red - Nanog; green - Gcna (TRA98; assay MGI:7484010).
Structure Level Pattern Image Note
TS24: germ cell of testis Absent 4B E16.5 , 4B E16.5 NANOG
Specimen 5A: postnatal week 8 (more )
Note: Double labeled: red - Nanog; green - Dppa3 (PGC7; assay MGI:3584539).
Structure Level Pattern Image Note
TS28: ovary Absent 5A NANOG , 5A
Specimen 5B: postnatal week 8 (more )
Note: Double labeled: red - Nanog; green - Gcna (TRA98; assay MGI:7484010).
Structure Level Pattern Image Note
TS28: testis Absent 5B , 5B NANOG
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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