Probe:	Fbln1 oligo
Probe preparation:	Antisense labelled with S35 DNA
Assay notes:	The probe used in this assay was 3' labelled.

Structure	Level	Pattern	Image	Note
TS17: embryo mesenchyme	Present	Regionally restricted		Expression is in mesenchymal cells in most parts of the body.
TS17: heart	Present	Regionally restricted		Expression is in the epicardium.
TS17: cardiac muscle tissue	Present
TS17: endocardial lining	Present
TS17: endocardial cushion	Weak

Structure	Level	Pattern	Image	Note
TS21: tongue	Strong		3A/B
TS21: central nervous system	Strong	Regionally restricted	3A/B
TS21: peripheral nervous system	Strong	Regionally restricted	3A/B
TS21: intestine	Strong	Regionally restricted	3A/B	Expression is in the epithelial cells of the mucosa and the mesenchymal cells of the submucosa, lower levels are in the muscle layer.
TS21: embryo mesenchyme	Present		3A/B
TS21: larynx aditus	Present		3A/B
TS21: lung mesenchyme	Present		3A/B
TS21: palatal shelf	Present		3A/B
TS21: primary palate	Present		3A/B
TS21: head mesenchyme	Present	Regionally restricted	3A/B	Expression is in tissues derived from the neural crest mesenchyme.
TS21: dorsal aorta	Strong	Regionally restricted	3A/B	Expression is in the smooth muscle.
TS21: vertebral cartilage condensation	Present	Regionally restricted	3A/B	Expression is in the perichondrium.
TS21: mesenchyme of metanephros	Present	Regionally restricted	3A/B	Expression is in all parts of the mesenchyme except for the condensed mesenchyme which surrounds the tip of the developing ureter bud.
TS21: diencephalon lateral wall marginal layer	Present		3A/B
TS21: diencephalon lateral wall ventricular layer	Present		3A/B
TS21: cerebellum	Present	Regionally restricted	3A/B	Expression is in the ependymal layer.
TS21: liver	Absent		3A/B

Structure	Level	Pattern	Image	Note
TS22: humerus cartilage condensation	Present	Regionally restricted	5A/B	Expression is in the perichondrium.
TS22: scapula cartilage condensation	Present	Regionally restricted	5A/B	Expression is in the perichondrium.

Structure	Level	Pattern	Image	Note
TS22: hindlimb phalanx cartilage condensation	Present	Regionally restricted	5E/F

Structure	Level	Pattern	Image	Note
TS21: temporal bone petrous part	Absent		6A/B
TS21: head mesenchyme	Present	Regionally restricted	6A/B	Expression is in the mesenchymal cells around the petrous part of temporal bone.

Structure	Level	Pattern	Image	Note
TS21: upper jaw incisor epithelium	Present		7A/B
TS21: upper jaw incisor mesenchyme	Present		7A/B
TS21: upper jaw mesenchyme	Weak		7A/B
TS21: oral epithelium	Strong		7A/B

Structure	Level	Pattern	Image	Note
TS26: nose	Strong	Regionally restricted	8A/B	Expression is in mesenchymal cells that surround the nasal cartilage.

Structure	Level	Pattern	Image	Note
TS21: oral epithelium	Present		9A/B
TS21: vibrissa dermal component	Present		9A/B
TS21: vibrissa epidermal component	Present		9A/B

Structure	Level	Pattern	Image	Note
TS26: vibrissa dermal component	Weak		9E/F
TS26: head mesenchyme	Weak		9E/F	Expression is between the vibrissae.

Structure	Level	Pattern	Image	Note
TS23: dental papilla	Very strong		11A/B
TS23: tooth epithelium	Present		11A/B

Structure	Level	Pattern	Image	Note
TS26: enamel organ	Very strong		11E/F
TS26: dental papilla	Present		11E/F

Structure	Level	Pattern	Image	Note
TS24: lung mesenchyme	Present	Regionally restricted	13C/D	Expression is in mesenchyme adjacent to each bronchus.
TS24: smooth muscle tissue	Present	Regionally restricted	13C/D	Expression is in smooth muscle surrounding blood vessels.

Structure	Level	Pattern	Image	Note
TS22: head mesenchyme	Present	Regionally restricted	14A/B	Expression is in the mesenchymal cells which extend under the ependymal layer of the choroid plexus.

Structure	Level	Pattern	Image	Note
TS22: spinal cord mantle layer	Weak		14E/F
TS22: spinal cord meninges	Present		14E/F

Structure	Level	Pattern	Image	Note
TS20: Meckel's cartilage	Absent

Structure	Level	Pattern	Image	Note
TS21: Meckel's cartilage	Absent
TS21: testis mesenchyme	Present	Regionally restricted		Expression is in the mesenchyme cells between the seminiferous tubules.

Structure	Level	Pattern	Image	Note
TS22: Meckel's cartilage	Absent

Structure	Level	Pattern	Image	Note
TS23: Meckel's cartilage	Absent

Structure	Level	Pattern	Image	Note
TS26: Meckel's cartilage	Absent