Gene Expression Data
Assay Details
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Assay
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Reference:
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J:91307
Korteweg N, et al., Development of the mouse hypothalamo-neurohypophysial system in the munc18-1 null mutant that lacks regulated secretion. Eur J Neurosci. 2004 Jun;19(11):2944-52
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3581519
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Gene symbol:
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Gap43
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Gene name:
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growth associated protein 43
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Antibody:
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9527
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3A:
embryonic day 14.5
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Stxbp1tm1Sud/Stxbp1tm1Sud
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: median eminence |
Present |
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3C
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Expression is present in fibers; there are fewer in mutants compared to the wild type.
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Specimen
3E:
embryonic day 14.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
3G:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Stxbp1tm1Sud/Stxbp1tm1Sud
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neurohypophysis pars nervosa |
Present |
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3G
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Expression is present in fibers in the neural lobe; there are fewer in mutants compared to wild type.
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Specimen
Wild type:
embryonic day 16.5
(more )
Specimen
Wild type:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
5G:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Stxbp1tm1Sud/Stxbp1tm1Sud
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Note:
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This is a coronal section.
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Specimen
Wild type:
embryonic day 16.5
(more )
Specimen
Wild type:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: neurohypophysis pars nervosa |
Present |
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Expression is present in neuronal fibers on the periphery of the neural lobe; there is no staining in the center of the lobe.
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Specimen
5H:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Stxbp1tm1Sud/Stxbp1tm1Sud
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Note:
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This is a coronal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: neurohypophysis pars nervosa |
Present |
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5H
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Expression is present in neuronal fibers on the periphery of the neural lobe; there is no staining in the center of the lobe.
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Specimen
7C:
embryonic day 18.5
(more )
Specimen
7C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
7E:
embryonic day 18.5
(more )
Specimen
7E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
7H:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Stxbp1tm1Sud/Stxbp1tm1Sud
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neurohypophysis infundibulum |
Present |
Regionally restricted |
7H
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There are occasional positive fibers in the pituitary stalk of the mutant.
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Specimen
7J:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Stxbp1tm1Sud/Stxbp1tm1Sud
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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