Gene Expression Data
Assay Details
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Assay
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Reference:
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J:96874
Tsuji D, et al., Specific induction of macrophage inflammatory protein 1-alpha in glial cells of Sandhoff disease model mice associated with accumulation of N-acetylhexosaminyl glycoconjugates. J Neurochem. 2005 Mar;92(6):1497-507
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3581056
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Gene symbol:
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Aif1
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Gene name:
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allograft inflammatory factor 1
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Antibody:
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anti-Iba1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3(m):
postnatal week 5
(more  )
Specimen
3(m):
(close  )
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Genetic Background:
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C57BL/6
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Age:
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postnatal week 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hippocampus |
Present |
Single cells |
3(m)
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Expression is in microglial cells.
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Specimen
3(n):
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Hexbtm1Rlp/Hexbtm1Rlp
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Age:
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postnatal week 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hippocampus |
Strong |
Single cells |
3(n)
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Expression is in microglial cells. An increase in expression is seen in this mutant when compared to wild-type.
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Specimen
3(o):
postnatal week 15
(more )
Specimen
3(o):
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal week 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hippocampus |
Present |
Single cells |
3(o)
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Expression is in microglial cells.
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Specimen
3(p):
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Hexbtm1Rlp/Hexbtm1Rlp
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Age:
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postnatal week 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hippocampus |
Strong |
Single cells |
3(p)
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Expression is in microglial cells. An increase in expression is seen in this mutant when compared to wild-type.
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Specimen
4(e):
postnatal week 15; Hexbtm1Rlp/ Hexbtm1Rlp
(more )
Note: Double labeled: brown - Aif1; red - GlcNAc.
Specimen
4(e):
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Hexbtm1Rlp/Hexbtm1Rlp
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Age:
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postnatal week 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: brown - Aif1; red - GlcNAc.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brainstem |
Strong |
Single cells |
4(e)
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Expression is in microglial cells.
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Analysis Tools