Gene Expression Data
Assay Details
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Assay
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Reference:
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J:97065
Asayesh A, et al., Developmental expression of metalloproteases ADAM 9, 10, and 17 becomes restricted to divergent pancreatic compartments. Dev Dyn. 2005 Apr;232(4):1105-14
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3580815
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Gene symbol:
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Adam9
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Gene name:
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ADAM metallopeptidase domain 9
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Antibody:
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anti-ADAM9
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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The images were confocal images.
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Results
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Specimen
1C, 1I:
embryonic day 10.0
(more  )
Note: Double labeled: red - Adam9; green - Gcg (assay MGI:3580821).
Specimen
1C, 1I:
(close  )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam9; green - Gcg (assay MGI:3580821).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: pancreas primordium |
Present |
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1C
,
1I
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Expression was detected in the dorsal and ventral pancreatic buds as well as in the intervening gut endoderm and pancreas-associated mesenchyme.
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Specimen
Table 1:
embryonic day 9.5
(more )
Specimen
Table 1:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: pancreas primordium |
Present |
Regionally restricted |
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Expression is present in the pancreatic epithelium, as well as the pancreas associated mesenchyme.
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Specimen
2G:
embryonic day 12.5
(more )
Specimen
2G:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pancreas |
Present |
Uniform |
2G
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Expression was uniformly distributed throughout the pancreas corresponding to both the Ptf1a-positive (exocrine) and Isl1-positive (endocrine) subpopulations.
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Specimen
2H:
embryonic day 15.5
(more )
Specimen
2H:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
Uniform |
2H
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Expression was uniformly distributed throughout the pancreas corresponding to both the Ptf1a-positive (exocrine) and Isl1-positive (endocrine) subpopulations.
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Specimen
2I:
embryonic day 17.5
(more )
Specimen
2I:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: pancreas |
Present |
Non-Uniform |
2I
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Expression was reduced in the exocrine compartment relative to the endocrine compartment.
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Specimen
Table 1:
embryonic day 14.5
(more )
Specimen
Table 1:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: pancreas |
Present |
Uniform |
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Expression was uniformly distributed throughout the pancreas.
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Specimen
Table 1:
embryonic day 16.5
(more )
Specimen
Table 1:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: pancreas |
Present |
Uniform |
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Expression was uniformly distributed throughout the pancreas.
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Specimen
3A:
postnatal day 1
(more )
Note: Double labeled: red - Adam9; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
Specimen
3A:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam9; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
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Specimen
3B:
postnatal day 1
(more )
Note: Double labeled: red - Adam9; green - Gcg (assay MGI:3580821).
Specimen
3B:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam9; green - Gcg (assay MGI:3580821).
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Specimen
3C:
postnatal day 1
(more )
Note: Double labeled: red - Adam9; green - Sst (assay MGI:3580819).
Specimen
3C:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam9; green - Sst (assay MGI:3580819).
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Specimen
4A:
postnatal week 8
(more )
Note: Double labeled: red - Adam9; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
Specimen
4A:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal week 8
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam9; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pancreas |
Present |
Regionally restricted |
4A
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Expression was restricted to the insulin-producing cells.
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Specimen
4B:
postnatal week 8
(more )
Note: Double labeled: red - Adam9; green - Gcg (assay MGI:3580821).
Specimen
4B:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal week 8
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam9; green - Gcg (assay MGI:3580821).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pancreas |
Present |
Regionally restricted |
4B
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Expression was excluded from the glucagon-expressing cells.
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Specimen
4C:
postnatal week 8
(more )
Note: Double labeled: red - Adam9; green - Sst (assay MGI:3580819).
Specimen
4C:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal week 8
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam9; green - Sst (assay MGI:3580819).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pancreas |
Present |
Regionally restricted |
4C
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Expression was excluded from the somatostatin-expressing cells.
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Specimen
5A, 5C:
postnatal adult
(more )
Note: Double labeled: red - Adam9; green - Cdh1 (assay MGI:3580832).
Specimen
5A, 5C:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam9; green - Cdh1 (assay MGI:3580832).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
Present |
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5C
,
5A
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Expression was detected in both the cytoplasm and the outer cell membrane of adult islets. Expression colocalized with Cdh1 (E-Cadherin) in the outer cell membrane.
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Specimen
6A, 6C:
embryonic day 15.5
(more )
Note: This specimen was also stained for DBA-lectin (green).
Specimen
6A, 6C:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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This specimen was also stained for DBA-lectin (green).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
Regionally restricted |
6C
,
6A
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Expression was detected in pancreatic ductal cells.
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Specimen
6J, 6L:
postnatal week 8
(more )
Note: This specimen was also stained for DBA-lectin (green).
Specimen
6J, 6L:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal week 8
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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This specimen was also stained for DBA-lectin (green).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pancreatic duct |
Present |
Regionally restricted |
6L
,
6J
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Expression was detected in the ductal epithelium.
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