Gene Expression Data
Assay Details
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Assay
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Reference:
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J:91734
Wang Y, et al., Entire mitogen activated protein kinase (MAPK) pathway is present in preimplantation mouse embryos. Dev Dyn. 2004 Sep;231(1):72-87
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3578372
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Gene symbol:
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Raf1
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Gene name:
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v-raf-leukemia viral oncogene 1
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Antibody:
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Phospho Raf1 (Ser 338) (SC-12358)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to fluorescein
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Assay notes:
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Antibody recognizes the phosphorylated form of the protein. The authors did not specify which antibody was used. They could have used: Phospho Raf1 (Ser 338) (SC-12358) (MGI:3578348) or Phospho-Raf (Ser259) (9421) (MGI:3577644).
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Results
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Specimen
5A,C:
embryonic day 3.5
(more  )
Note: Morula/early cavitation blastocyst.
Specimen
5A,C:
(close  )
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Genetic Background:
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involves: C57BL/6J * MF1 * SJL/J
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Morula/early cavitation blastocyst.
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Specimen
6A,C:
embryonic day 2.5
(more )
Specimen
6A,C:
(close )
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Genetic Background:
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involves: C57BL/6J * MF1 * SJL/J
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Age:
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embryonic day 2.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Analysis Tools
