Antibody:	Anti-Fibronectin
Detection system:	Secondary antibody
Assay notes:	The fixative was 0.2% paraformaldehyde in 0.1M phosphate buffer with 0.12mM CaCl2, 4% sucrose.

Structure	Level	Pattern	Image	Note
TS19: trunk mesenchyme	Strong	Regionally restricted	1G	Expression was strong in the mesenchyme around the myotome.
TS19: trunk dermomyotome	Present	Regionally restricted	1G	Expression was detected in a dotty pattern inside the myotome.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present		1P

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS20: body wall mesenchyme	Present	Regionally restricted	2H	Expression was detected around the Myog (Myogenin) positive myogenic cells in abdominal wall muscle masses.

Structure	Level	Pattern	Image	Note
TS22: vertebral axis musculature	Present	Regionally restricted	2P	Expression was detected around myogenic cells.

Structure	Level	Pattern	Image	Note
TS22: vertebral axis musculature	Present	Regionally restricted	2P Inset	Expression was detected around myogenic cells.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	3H	Expression was present in a matrix surrounding all non-myogenic and myogenic Myog (myogenin) positive cells.

Structure	Level	Pattern	Image	Note
TS22: forelimb mesenchyme	Present		3L
TS22: forelimb musculature	Present	Regionally restricted	3L	Expression was detected around the myotubes and is less intense than in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: forelimb musculature	Present	Regionally restricted	3L Inset	Expression was detected in the myotubes.