Antibody:	PS-2
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	The fixative was 0.2% paraformaldehyde in 0.1M phosphate buffer with 0.12mM CaCl2, 4% sucrose.

Structure	Level	Pattern	Image	Note
TS19: trunk dermomyotome	Strong	Regionally restricted	1E	Expression was strong in myotome.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	1M	Expression was detected in dorsal and ventral muscle masses.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Regionally restricted		Expression was detected in dorsal and ventral muscle masses.

Structure	Level	Pattern	Image	Note
TS20: body wall mesenchyme	Present	Regionally restricted	2E	Expression was detected in abdominal wall muscle masses.

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Present	Regionally restricted	2O	Expression was detected in trunk muscles.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	3E	Expression was detected in dorsal and ventral muscle masses.

Structure	Level	Pattern	Image	Note
TS22: forelimb musculature	Present	Regionally restricted	3J	Expression was detected in muscle masses.