Antibody:	anti-alpha6 integrin (GoH3)
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	The fixative was 0.2% paraformaldehyde in 0.1M phosphate buffer with 0.12mM CaCl2, 4% sucrose.

Structure	Level	Pattern	Image	Note
TS19: trunk dermomyotome	Present	Regionally restricted	1A	Expression was detected widely in the myotome.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	1I	Expression was strong in the epidermis but was absent in forelimb myogenic cells.
TS19: blood vessel	Strong		1I

Structure	Level	Pattern	Image	Note
TS19: head	Strong	Regionally restricted		Expression was detected in the developing epidermis.
TS19: trunk	Strong	Regionally restricted		Expression was detected in the developing epidermis.

Structure	Level	Pattern	Image	Note
TS20: blood vessel	Strong		2A
TS20: epidermis	Strong		2A
TS20: body wall mesenchyme	Absent		2A	Expression was absent from muscle masses in the abdominal wall.

Structure	Level	Pattern	Image	Note
TS21: pectoral girdle and thoracic body wall muscle	Present	Regionally restricted	2I	Expression was detected in trunk muscles and was most evident in the intercostal, pectoral and deep back muscle cells.
TS21: vertebral axis musculature	Present	Regionally restricted	2I	Expression was detected in trunk muscles and was most evident in the intercostal, pectoral and deep back muscle cells.

Structure	Level	Pattern	Image	Note
TS22: axial muscle	Present	Regionally restricted	2M	Expression was detected in the intercostal muscle in the primary myotubes.
TS22: pectoral girdle and thoracic body wall muscle	Present	Regionally restricted	2M	Expression was detected in the pectoral muscle in the primary myotubes.

Structure	Level	Pattern	Image	Note
TS20: blood vessel	Strong		3A
TS20: epidermis	Strong		3A
TS20: forelimb mesenchyme	Absent		3A	Expression was absent from the forelimb muscle masses.

Structure	Level	Pattern	Image	Note
TS22: hindlimb musculature	Weak	Regionally restricted	3I	Expression was weak in the muscle masses.