Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:74049
Segat D, et al., Expression of matrilin-1, -2 and -3 in developing mouse limbs and heart. Matrix Biol. 2000 Dec;19(7):649-55
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:3576371
|
|
Gene symbol:
|
Matn3
|
|
Gene name:
|
matrilin 3
|
|
Antibody:
|
anti-matrilin 3
|
|
Detection system:
|
Secondary antibody coupled to Cy3
|
|
Assay notes:
|
The tissue was frozen, sectioned, and then fixed with 1% paraformaldehyde in PBS for 10 minutes.
|
|
|
Results
|
Specimen
1 9.5 dpc:
embryonic day 10.0
(more  )
Specimen
1 9.5 dpc:
(close  )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: heart |
Absent |
|
1_9.5 dpc Matn3
|
|
Specimen
1 10.5 dpc:
embryonic day 11.0
(more )
Specimen
1 10.5 dpc:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: heart |
Absent |
|
1_10.5 dpc Matn3
|
|
Specimen
3 12.5 dpc:
embryonic day 13.0
(more )
Specimen
3 12.5 dpc:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 13.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
E12.5--not shown:
embryonic day 13.0
(more )
Specimen
E12.5--not shown:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 13.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3 13.5 dpc:
embryonic day 14.0
(more )
Specimen
3 13.5 dpc:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3 14.5 dpc:
embryonic day 15.0
(more )
Specimen
3 14.5 dpc:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 15.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: forelimb skeleton |
Present |
|
3_14.5 dpc Matn3
|
Expression is present in the cartilage.
|
Specimen
4:
embryonic day 15.0
(more )
Specimen
4:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 15.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: humerus |
Present |
|
4_Matn3
|
Expression is present in the elbow, in the zones of proliferating and early hypertrophic chondrocytes.
|
| TS23: ulna |
Present |
|
4_Matn3
|
Expression is present in the elbow, in the zones of proliferating and early hypertrophic chondrocytes.
|
Specimen
5 15.5 dpc:
embryonic day 16.0
(more )
Specimen
5 15.5 dpc:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 16.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: forelimb skeleton |
Present |
|
5_15.5 dpc Matn3
|
Expression is present in the developing bone anlage.
|
Specimen
5 17.5 dpc left:
embryonic day 18.0
(more )
Specimen
5 17.5 dpc left:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forelimb skeleton |
Present |
|
5_17.5 dpc Matn3 left
|
Expression is present in the developing bone anlage.
|
Specimen
5 17.5 dpc right:
embryonic day 18.0
(more )
Specimen
5 17.5 dpc right:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forelimb skeleton |
Present |
|
5_17.5 dpc Matn3 right
|
Expression is present in the developing bone anlage.
|
Specimen
6:
embryonic day 16.0
(more )
Specimen
6:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 16.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: elbow joint |
Present |
Non-Uniform |
6_Matn3
|
Expression is not as intensive at the articular surface as it is in the deeper zone of proliferating cells.
|
| TS24: humerus |
Present |
Non-Uniform |
6_Matn3
|
Expression is not as intensive at the articular surface as it is in the deeper zone of proliferating cells.
|
| TS24: ulna |
Present |
Non-Uniform |
6_Matn3
|
Expression is not as intensive at the articular surface as it is in the deeper zone of proliferating cells.
|
|
Analysis Tools