Probe:	clone 1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		1A/B
TS13: extraembryonic component	Present	Regionally restricted	1A/B	Expression is in the trophoblastic giant cells, absent in the amnion, allantois, and chorion.
TS13: yolk sac	Absent		1A/B

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		2A/B

Structure	Level	Pattern	Image	Note
TS15: notochord	Present	Graded	2E	The strongest signal was around the 12th pair of somites.

Structure	Level	Pattern	Image	Note
TS20: notochord	Present
TS20: pre-cartilage condensation	Absent

Structure	Level	Pattern	Image	Note
TS21: cartilage	Strong
TS21: limb digit	Weak	Patchy		Expression is in the 1-cell deep suprabasal layer of the epidermis.
TS21: head	Weak	Patchy		Expression is in the 1-cell deep suprabasal layer of the epidermis on the muzzle.

Structure	Level	Pattern	Image	Note
TS22: cranium	Present		3A
TS22: Meckel's cartilage	Strong		3A
TS22: exoccipital cartilage condensation	Strong		3A
TS22: temporal bone	Strong		3A
TS22: rib cartilage condensation	Present		3A
TS22: vertebral cartilage condensation	Present		3A
TS22: cervical intervertebral disc	Absent		3A

Structure	Level	Pattern	Image	Note
TS22: vertebral cartilage condensation	Present	Graded	3B/C	Expression is stronger in the outer cells (hypertrophic chondrocytes).

Structure	Level	Pattern	Image	Note
TS22: humerus cartilage condensation	Strong	Regionally restricted	3D/E	Moderate expression in cartilage and very high levels in the transition zone (hypertrophic chondrocytes). Absent in osteoblasts.

Structure	Level	Pattern	Image	Note
TS23: epidermis	Strong	Regionally restricted	4A/B	Expression is in the suprabasal layers and in the central cells of the hair follicles.
TS23: dermis	Absent		4A/B

Structure	Level	Pattern	Image	Note
TS27: epidermis	Present	Regionally restricted		Expression is in the suprabasal layers.
TS27: dermis	Absent

Structure	Level	Pattern	Image	Note
TS28: epidermis	Present	Regionally restricted		Expression is in the basal and suprabasal layers.
TS28: dermis	Absent
TS28: hair follicle	Present	Regionally restricted		Expression is in the inner root sheath of hair follicles.

Structure	Level	Pattern	Image	Note
TS19: liver	Present	Single cells

Structure	Level	Pattern	Image	Note
TS20: liver	Weak	Single cells	5A/B

Structure	Level	Pattern	Image	Note
TS22: lung	Trace	Single cells	5C/D

Structure	Level	Pattern	Image	Note
TS22: bladder	Present	Regionally restricted	5E/F	Expression is in the endodermal lining of the bladder.

Structure	Level	Pattern	Image	Note
TS22: larynx mucous membrane	Present		5G/H

Structure	Level	Pattern	Image	Note
TS22: liver	Present	Single cells
TS22: skeleton	Strong	Single cells		Expression is in 2-3 cells infiltrating the mineralizing part of the bones.
TS22: central nervous system	Absent
TS22: heart	Absent
TS22: intestine	Absent
TS22: metanephros	Absent
TS22: skeletal muscle	Absent
TS22: testis	Absent

Structure	Level	Pattern	Image	Note
TS23: esophagus	Present	Regionally restricted		Expression is in the lining.
TS23: liver	Present	Single cells
TS23: skeleton	Present	Single cells		Expression is in 2-3 cells infiltrating the mineralizing part of the bones.