Probe:	Vn cDNA
Probe preparation:	Antisense RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: hepatic primordium	Strong		1C , 1A/B
TS17: stomach epithelium	Strong		1C , 1A/B
TS17: heart	Absent		1A/B , 1C

Structure	Level	Pattern	Image	Note
TS21: liver	Strong		1D/E , 1F
TS21: blood	Absent		1F , 1D/E

Structure	Level	Pattern	Image	Note
TS24: liver	Strong	Regionally restricted	1I , 1G/H	Expression concentrates around hepatic blood vessels.

Structure	Level	Pattern	Image	Note
TS26: liver	Present	Uniform	1L , 1J/K
TS26: liver vasculature	Absent		1L , 1J/K	Absent in the endothelium and vessel wall of vessels in the liver.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Strong		2A/B , 2C
TS17: notochord	Strong		2A/B , 2C

Structure	Level	Pattern	Image	Note
TS20: notochord	Absent
TS20: spinal cord floor plate	Absent

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	2F , 2D/E	Expression is in the meninges.
TS20: cardiovascular system	Present	Regionally restricted	2F , 2D/E	Expression is in the invading capillaries in the brain.

Structure	Level	Pattern	Image	Note
TS26: olfactory lobe	Present		2G , 2H/I

Structure	Level	Pattern	Image	Note
TS26: pons	Present		2J/K , 2L

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted		Expression is in the meninges.

Structure	Level	Pattern	Image	Note
TS23: cardiovascular system	Present	Regionally restricted	3A/B , 3C	Expression is in capillaries of the pia mater.
TS23: diencephalon pia mater	Present	Regionally restricted	3A/B , 3C	Expression is in capillaries of the pia mater.
TS23: hindbrain pia mater	Present	Regionally restricted	3A/B , 3C	Expression is in capillaries of the pia mater.
TS23: midbrain pia mater	Present	Regionally restricted	3A/B , 3C	Expression is in capillaries of the pia mater.
TS23: telencephalon pia mater	Present	Regionally restricted	3A/B , 3C	Expression is in capillaries of the pia mater.
TS23: brain dura mater	Absent		3C , 3A/B
TS23: diencephalon arachnoid mater	Absent		3C , 3A/B
TS23: hindbrain arachnoid mater	Absent		3C , 3A/B
TS23: midbrain arachnoid mater	Absent		3C , 3A/B
TS23: telencephalon arachnoid mater	Absent		3C , 3A/B

Structure	Level	Pattern	Image	Note
TS26: peripheral nervous system	Present	Regionally restricted	3F , 3D/E	Expression is in Schwann cells.

Structure	Level	Pattern	Image	Note
TS26: peripheral nervous system	Present	Regionally restricted	3G/H , 3I	Expression is in satellite cells of the motor and sensory ganglia.

Structure	Level	Pattern	Image	Note
TS17: blood vessel	Present	Regionally restricted	4A/B	Expression in blood vessels surrounding the neural tube.

Structure	Level	Pattern	Image	Note
TS24: blood vessel	Present	Regionally restricted	4C	Expression is in endothelial-like cells in a blood vessel in the brain.

Structure	Level	Pattern	Image	Note
TS21: brain meninges	Present		4D/E

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	4F	Expression is in mural cells and in endothelial-like cells in a blood vessel in the meninges.
TS20: cardiovascular system	Present	Regionally restricted	4F	Expression is in mural cells and in endothelial-like cells in a blood vessel in the meninges.

Structure	Level	Pattern	Image	Note
TS26: blood vessel	Present	Regionally restricted	4I , 4G , 4H	Expression is in mural cells in the meninges.
TS26: brain meninges	Present	Regionally restricted	4I , 4G , 4H	Expression is in mural cells in the meninges.

Structure	Level	Pattern	Image	Note
TS26: blood vessel endothelium	Present		4J	Expression is in the endothelium.

Structure	Level	Pattern	Image	Note
TS26: choroid plexus	Absent		4K	Absent in blood vessels in the choroid plexus.

Structure	Level	Pattern	Image	Note
TS26: skin	Absent		4L	Absent in blood vessels in the skin.

Structure	Level	Pattern	Image	Note
TS17: midgut	Present	Regionally restricted	5A/B	Expression is in the future epithelium.

Structure	Level	Pattern	Image	Note
TS17: stomach epithelium	Present		5C

Structure	Level	Pattern	Image	Note
TS20: midgut	Absent			Expression is absent in the epithelium.
TS20: stomach	Absent			Expression is absent in the epithelium.

Structure	Level	Pattern	Image	Note
TS17: trunk dermomyotome	Present		5D

Structure	Level	Pattern	Image	Note
TS17: trunk dermomyotome	Present	Single cells	5E

Structure	Level	Pattern	Image	Note
TS17: trunk mesenchyme	Present	Regionally restricted	5F	Expression is in mesenchymal cells that surround the developing dorsal ganglion.

Structure	Level	Pattern	Image	Note
TS17: brain	Present			Expression is in the brain and brain blood vessels, absent in the meninges and nerves/ganglia.
TS17: nervous system	Present			Expression is in the brain and brain blood vessels, absent in the meninges and nerves/ganglia.
TS17: ear	Absent
TS17: eye	Absent
TS17: heart	Absent
TS17: lung	Absent
TS17: stomach	Absent
TS17: surface ectoderm	Absent
TS17: blood vessel	Present	Regionally restricted		Expression is in the brain blood vessels, absent in large vessels and capillaries.
TS17: genitourinary system	Absent			Absent in the kidney.
TS17: liver	Present
TS17: gut	Present	Regionally restricted		Expression is in the primitive endoderm.

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and in the meninges and is absent in nerves/ganglia.
TS19: nervous system	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and in the meninges and is absent in nerves/ganglia.
TS19: ear	Absent
TS19: eye	Absent
TS19: gut	Absent
TS19: heart	Absent
TS19: lung	Absent
TS19: metanephros	Absent
TS19: stomach	Absent
TS19: surface ectoderm	Absent
TS19: blood vessel	Present	Regionally restricted		Expression is in the brain blood vessels, absent in large vessels and capillaries.
TS19: liver	Present

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and in the meninges and is absent in nerves/ganglia.
TS20: nervous system	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and in the meninges and is absent in nerves/ganglia.
TS20: ear	Absent
TS20: eye	Absent
TS20: gut	Absent
TS20: heart	Absent
TS20: integumental system	Absent
TS20: lung	Absent
TS20: metanephros	Absent
TS20: stomach	Absent
TS20: blood vessel	Present	Regionally restricted		Expression is in the brain blood vessels, absent in large vessels and capillaries.
TS20: liver	Present

Structure	Level	Pattern	Image	Note
TS21: brain	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and is absent in nerves/ganglia.
TS21: nervous system	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and is absent in nerves/ganglia.
TS21: brain meninges	Present
TS21: liver	Present
TS21: ear	Absent
TS21: eye	Absent
TS21: gut	Absent
TS21: heart	Absent
TS21: integumental system	Absent
TS21: lung	Absent
TS21: metanephros	Absent
TS21: stomach	Absent
TS21: blood vessel	Present	Regionally restricted		Expression is in the brain blood vessels, absent in large vessels and capillaries.

Structure	Level	Pattern	Image	Note
TS23: brain	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and is absent in nerves/ganglia.
TS23: nervous system	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and is absent in nerves/ganglia.
TS23: brain meninges	Present
TS23: liver	Present
TS23: dermis	Absent
TS23: ear	Absent
TS23: epidermis	Absent
TS23: eye	Absent
TS23: gut	Absent
TS23: heart	Absent
TS23: lung	Absent
TS23: metanephros	Absent
TS23: stomach	Absent
TS23: blood vessel	Present	Regionally restricted		Expression is in the brain blood vessels, absent in large vessels and capillaries.

Structure	Level	Pattern	Image	Note
TS24: brain	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and in nerves/ganglia.
TS24: nervous system	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and in nerves/ganglia.
TS24: brain meninges	Present
TS24: liver	Present
TS24: dermis	Absent
TS24: ear	Absent
TS24: epidermis	Absent
TS24: eye	Absent
TS24: gut	Absent
TS24: heart	Absent
TS24: lung	Absent
TS24: metanephros	Absent
TS24: stomach	Absent
TS24: blood vessel	Present	Regionally restricted		Expression is in the brain blood vessels, absent in large vessels and capillaries.

Structure	Level	Pattern	Image	Note
TS25: brain	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and in nerves/ganglia.
TS25: nervous system	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and in nerves/ganglia.
TS25: brain meninges	Present
TS25: ear	Present
TS25: liver	Present
TS25: dermis	Absent
TS25: epidermis	Absent
TS25: eye	Absent
TS25: gut	Absent
TS25: heart	Absent
TS25: lung	Absent
TS25: metanephros	Absent
TS25: stomach	Absent
TS25: blood vessel	Present	Regionally restricted		Expression is in the brain blood vessels, absent in large vessels and capillaries.

Structure	Level	Pattern	Image	Note
TS26: brain	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and in nerves/ganglia.
TS26: nervous system	Present	Regionally restricted		Expression is in the brain, brain blood vessels, and in nerves/ganglia.
TS26: brain meninges	Present
TS26: ear	Present
TS26: liver	Present
TS26: dermis	Absent
TS26: epidermis	Absent
TS26: eye	Absent
TS26: gut	Absent
TS26: heart	Absent
TS26: lung	Absent
TS26: metanephros	Absent
TS26: stomach	Absent
TS26: blood vessel	Present	Regionally restricted		Expression is in the brain blood vessels, absent in large vessels and capillaries.