Gene Expression Data
Assay Details
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Assay
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Reference:
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J:94054
Sakai Y, et al., Co-expression of de novo DNA methyltransferases Dnmt3a2 and Dnmt3L in gonocytes of mouse embryos. Gene Expr Patterns. 2004 Dec;5(2):231-7
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3528696
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Gene symbol:
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Dnmt3a
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Gene name:
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DNA methyltransferase 3A
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Antibody:
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anti-Dnmt3a
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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All the samples were simultaneously stained with TRA98 (MGI:5009424) to identify gonocytes and spermatogonia.
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Results
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Specimen
2A:
embryonic day 14.0
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: seminiferous cord |
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Present |
Regionally restricted |
2A
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Staining is in the nuclei of the Sertoli cells and connective tissue cells that surround gonocytes or spermatogonia.
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| TS22: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
2A
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Staining is in the nuclei of the Sertoli cells and connective tissue cells that surround gonocytes or spermatogonia.
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| TS22: germ cell of testis |
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Absent |
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2A
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Specimen
2E:
embryonic day 16.0
(more )
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: seminiferous cord |
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Present |
Regionally restricted |
2E
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Staining is in the nuclei of the Sertoli cells and connective tissue cells that surround gonocytes or spermatogonia.
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| TS24: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
2E
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Staining is in the nuclei of the Sertoli cells and connective tissue cells that surround gonocytes or spermatogonia.
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| TS24: germ cell of testis |
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Absent |
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2E
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Specimen
2I:
embryonic day 18.0
(more )
Specimen
2I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: seminiferous cord |
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Present |
Regionally restricted |
2I
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Staining is in the nuclei of the Sertoli cells and connective tissue cells that surround gonocytes or spermatogonia.
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| TS26: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
2I
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Staining is in the nuclei of the Sertoli cells and connective tissue cells that surround gonocytes or spermatogonia.
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| TS26: germ cell of testis |
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Absent |
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2I
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Specimen
2M:
postnatal day 2
(more )
Specimen
2M:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 2
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: seminiferous tubule |
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Present |
Regionally restricted |
2M
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Staining is in the nuclei of the Sertoli cells and connective tissue cells that surround gonocytes or spermatogonia and is absent in the germ cells.
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| TS27: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
2M
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Staining is in the nuclei of the Sertoli cells and connective tissue cells that surround gonocytes or spermatogonia and is absent in the germ cells.
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| TS27: germ cell of testis |
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Absent |
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2M
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Specimen
2Q:
postnatal day 4
(more )
Specimen
2Q:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 4
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: seminiferous tubule |
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Present |
Regionally restricted |
2Q
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Staining is in the nuclei of the Sertoli cells and connective tissue cells that surround gonocytes or spermatogonia and is absent in the germ cells.
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| TS28: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
2Q
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Staining is in the nuclei of the Sertoli cells and connective tissue cells that surround gonocytes or spermatogonia and is absent in the germ cells.
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| TS28: germ cell of testis |
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Absent |
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2Q
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Specimen
2U:
postnatal day 6
(more )
Specimen
2U:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 6
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: seminiferous tubule |
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Present |
Regionally restricted |
2U
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Staining is in the nuclei of the Sertoli cells and connective tissue cells that surround gonocytes or spermatogonia and is absent in the germ cells.
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| TS28: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
2U
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Staining is in the nuclei of the Sertoli cells and connective tissue cells that surround gonocytes or spermatogonia and is absent in the germ cells.
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| TS28: germ cell of testis |
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Absent |
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2U
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