Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:59925
Miner JH, et al., Defective glomerulogenesis in the absence of laminin alpha5 demonstrates a developmental role for the kidney glomerular basement membrane. Dev Biol. 2000 Jan 15;217(2):278-89
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:3528028
|
Gene symbol:
|
Nid1
|
Gene name:
|
nidogen 1
|
Antibody:
|
anti-entactin
|
Detection system:
|
Secondary antibody
|
Assay notes:
|
Embryos were not fixed prior to sectioning. Authors state that sections were then sometimes fixed in acetone, ethanol or 2% paraformaldehyde.
|
|
Results
|
Specimen
6K:
embryonic day 16.5
(more )
Specimen
6K:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
6L:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Mutant Allele(s):
|
Lama5tm1Jhm/Lama5tm1Jhm
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: maturing glomerular tuft |
Present |
Regionally restricted |
6L
|
Authors report expression in mutant glomerular basement membrane; also associated with extruded endothelial and mesangial cells.
|
|