Gene Expression Data
Assay Details
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Assay
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Reference:
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J:59925
Miner JH, et al., Defective glomerulogenesis in the absence of laminin alpha5 demonstrates a developmental role for the kidney glomerular basement membrane. Dev Biol. 2000 Jan 15;217(2):278-89
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3528023
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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MEC 13.3
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Detection system:
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Secondary antibody
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Assay notes:
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Embryos were not fixed prior to sectioning. Authors state that sections were then sometimes fixed in acetone, ethanol or 2% paraformaldehyde.
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Results
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Specimen
5A:
embryonic day 16.5
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: maturing glomerular tuft |
Present |
Regionally restricted |
5A
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Authors report that, in constricting glomeruli, endothelial cells line the interior adjacent to the podocytes.
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Lama5tm1Jhm/Lama5tm1Jhm
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: maturing glomerular tuft |
Present |
Regionally restricted |
5B
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Authors report that, in constricting glomeruli, endothelial cells line the interior adjacent to the podocytes.
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Specimen
5C:
embryonic day 16.5
(more )
Note: Authors state that sections are at a later stage than sections shown in Figs. 5A and 5B.
Specimen
5C:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Authors state that sections are at a later stage than sections shown in Figs. 5A and 5B.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: maturing glomerular tuft |
Present |
Regionally restricted |
5C
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Expression in endothelial cells forming glomerular capillaries.
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Specimen
5D:
embryonic day 16.5; Lama5tm1Jhm/ Lama5tm1Jhm
(more )
Note: Authors state that sections are at a later stage than sections shown in Figs. 5A and 5B.
Specimen
5D:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Lama5tm1Jhm/Lama5tm1Jhm
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Authors state that sections are at a later stage than sections shown in Figs. 5A and 5B.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: maturing glomerular tuft |
Present |
Regionally restricted |
5D
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Authors report that endothelial cells are in the process of being extruded from within the constricting glomerulus.
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Analysis Tools