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Gene Expression Data
Assay Details
Assay
Reference: J:59925 Miner JH, et al., Defective glomerulogenesis in the absence of laminin alpha5 demonstrates a developmental role for the kidney glomerular basement membrane. Dev Biol. 2000 Jan 15;217(2):278-89
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3528018
Gene symbol: Wt1
Gene name: WT1 transcription factor
Antibody: anti-WT1 (C-19; sc-192)
Detection system: Secondary antibody
Assay notes: Embryos were not fixed prior to sectioning. Authors state that sections were then sometimes fixed in acetone, ethanol or 2% paraformaldehyde.
Results
Specimen 1A: embryonic day 10.75 (more )
Structure Level Pattern Image Note
TS18: metanephric mesenchyme Present 1A
Specimen 5A: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: maturing glomerular tuft Present Regionally restricted 5A Authors report expression in podocytes.
Specimen 5B: embryonic day 16.5; Lama5tm1Jhm/Lama5tm1Jhm (more )
Structure Level Pattern Image Note
TS24: maturing glomerular tuft Present Regionally restricted 5B Authors report expression in podocytes.
Specimen 5C: embryonic day 16.5 (more )
Note: Authors state that sections are at a later stage than sections shown in Figs. 5A and 5B.
Structure Level Pattern Image Note
TS24: maturing glomerular tuft Present Regionally restricted 5C Podocytes are arranged in a single-cell layer epithelium adjacent to glomerular capillaries formed by endothelial cells.
Specimen 5D: embryonic day 16.5; Lama5tm1Jhm/Lama5tm1Jhm (more )
Note: Authors state that sections are at a later stage than sections shown in Figs. 5A and 5B.
Structure Level Pattern Image Note
TS24: maturing glomerular tuft Present Regionally restricted 5D Authors report that podocytes are in disarray.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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