Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:95433
Vrljicak P, et al., Smad expression during kidney development. Am J Physiol Renal Physiol. 2004 Apr;286(4):F625-33
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3527861
|
|
Gene symbol:
|
Smad3
|
|
Gene name:
|
SMAD family member 3
|
|
Probe:
|
Smad3 cDNA3
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
whole mount:
embryonic day 15.0
(more  )
Specimen
whole mount:
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 15.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: metanephros |
Present |
Regionally restricted |
3_Smad3 whole-mount
|
|
Specimen
cryosection:
embryonic day 15.0
(more )
Specimen
cryosection:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 15.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: mesenchyme of metanephros |
Present |
Regionally restricted |
3_Smad3 cryosection
|
Expressed throughout the kidney in mesenchymal cells in both the medullary interstitium and the nephrogenic zone, and at ureteric bud tips, except for condensing mesenchymal structures where expression was downregulated.
|
| TS23: renal medullary interstitium |
Present |
Regionally restricted |
3_Smad3 cryosection
|
Expressed throughout the kidney in mesenchymal cells in both the medullary interstitium and the nephrogenic zone, and at ureteric bud tips, except for condensing mesenchymal structures where expression was downregulated.
|
| TS23: ureter |
Present |
Regionally restricted |
3_Smad3 cryosection
|
Expressed throughout the kidney in mesenchymal cells in both the medullary interstitium and the nephrogenic zone, and at ureteric bud tips, except for condensing mesenchymal structures where expression was downregulated.
|
Specimen
not shown:
embryonic day 12.0
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 12.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metanephros |
Present |
Regionally restricted |
|
Authors report similar results to that obtained at E15.
|
|
Analysis Tools