Gene Expression Data
Assay Details
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Assay
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Reference:
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J:95433
Vrljicak P, et al., Smad expression during kidney development. Am J Physiol Renal Physiol. 2004 Apr;286(4):F625-33
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3527855
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Gene symbol:
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Bmp4
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Gene name:
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bone morphogenetic protein 4
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Probe:
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Bmp4 cDNA20
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
whole mount:
embryonic day 15.0
(more  )
Specimen
whole mount:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: renal cortex |
Present |
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2_Bmp4 whole-mount
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Authors state that expression was detected mostly in the cortex.
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Specimen
cryosection:
embryonic day 15.0
(more )
Specimen
cryosection:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: mesenchyme of metanephros |
Present |
Regionally restricted |
2_Bmp4 cryosection
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Strongly expressed by uninduced mesenchymal cells in the nephrogenic zone. Fainter signal within the medullary interstitial compartment. Expressed in the mesenchyme adjacent to the ureteric bud tips and in the tips themselves.
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| TS23: renal medullary interstitium |
Present |
Regionally restricted |
2_Bmp4 cryosection
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Strongly expressed by uninduced mesenchymal cells in the nephrogenic zone. Fainter signal within the medullary interstitial compartment. Expressed in the mesenchyme adjacent to the ureteric bud tips and in the tips themselves.
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| TS23: ureter |
Present |
Regionally restricted |
2_Bmp4 cryosection
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Strongly expressed by uninduced mesenchymal cells in the nephrogenic zone. Fainter signal within the medullary interstitial compartment. Expressed in the mesenchyme adjacent to the ureteric bud tips and in the tips themselves.
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Specimen
not shown:
embryonic day 12.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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Not Specified
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metanephros |
Present |
Regionally restricted |
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Authors report similar results to that obtained at E15.
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Analysis Tools