Antibody:	anti-Foxa2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: respiratory tract	Present		1E	Expression is in epithelial cells of conducting and peripheral airways and alveoli.

Structure	Level	Pattern	Image	Note
TS28: adipose tissue	Absent
TS28: adrenal gland	Absent
TS28: arterial system	Absent
TS28: bladder	Absent
TS28: bone	Absent
TS28: capillary	Absent
TS28: cartilage	Absent
TS28: cerebellum Purkinje cell layer	Absent
TS28: esophagus	Absent
TS28: gallbladder epithelium	Absent
TS28: heart	Absent
TS28: main olfactory bulb	Absent
TS28: nasal cavity respiratory epithelium	Absent
TS28: parotid gland	Absent
TS28: renal cortex	Absent
TS28: renal medulla	Absent
TS28: skeletal muscle	Absent
TS28: smooth muscle tissue	Absent
TS28: spleen	Absent
TS28: submandibular gland	Absent
TS28: thymus	Absent
TS28: venous system	Absent
TS28: tongue	Present	Regionally restricted		Expression is present in the serous glands and is absent in the dorsal epithelium, mucous glands, and in the ventral epithelium.
TS28: stomach	Present	Regionally restricted		Expression is in the gastric glands and is absent in the stratified squamous epithelium.
TS28: large intestine	Present	Regionally restricted		Expression is in the crypts of Lieberkuhn and is absent in the single columnar epithelium.
TS28: small intestine	Present	Regionally restricted		Expression is in the crypts of Lieberkuhn and is absent in the single columnar epithelium.
TS28: liver	Present	Regionally restricted		Expression is in the hepatocytes and bile ducts.
TS28: islets of Langerhans	Present
TS28: pancreas	Present
TS28: olfactory epithelium	Present
TS28: olfactory gland	Absent
TS28: pharynx	Present	Regionally restricted		Expression is in the mucous acini and is absent in the columnar epithelium.
TS28: trachea	Present	Regionally restricted		Expression is in the stratified squamous epithelium and is absent in the tracheal glands.
TS28: bronchus	Present	Regionally restricted		Expression is in the bronchiolar epithelium and the alveolar epithelium (type II cells).
TS28: thyroid gland	Present

Structure	Level	Pattern	Image	Note
TS28: epididymis	Absent
TS28: penis	Absent
TS28: prostate gland	Absent
TS28: prostatic urethra	Absent
TS28: testis	Absent
TS28: anterior prostatic epithelium	Absent			coagulating gland
TS28: epithelium of seminal vesicle	Absent			vesicular gland

Structure	Level	Pattern	Image	Note
TS28: uterine gland	Present
TS28: mammary gland	Absent
TS28: ovary	Absent
TS28: vagina squamous epithelium	Absent
TS28: uterine horn epithelium	Absent			endometrium
TS28: endometrium luminal epithelium	Absent			uterus columnar epithelium

Structure	Level	Pattern	Image	Note
TS17: hepatic primordium	Present		2B
TS17: hindgut	Present		2B
TS17: laryngo-tracheal groove	Present		2B
TS17: lung	Present		2B
TS17: neural tube	Present		2B
TS17: notochord	Present		2B
TS17: pharynx	Present		2B
TS17: tail neural tube	Present		2B

Structure	Level	Pattern	Image	Note
TS21: liver	Present		2D
TS21: lung	Present		2D
TS21: stomach	Present		2D
TS21: tongue epithelium	Present	Regionally restricted	2D	Expression is in the dorsal epithelium.
TS21: midbrain	Present	Regionally restricted	2D	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS23: esophagus	Present		3E
TS23: intestine	Present		3E
TS23: liver	Present		3E
TS23: lung	Present		3E
TS23: pancreas	Present		3E
TS23: stomach	Present		3E
TS23: tongue epithelium	Present	Regionally restricted	3E	Expression is in the dorsal epithelium.
TS23: spinal cord floor plate	Present	Regionally restricted	3E	Expression is at the level of the fore- and hindlimb.
TS23: midbrain	Present	Regionally restricted	3E	Expression is in the ventral midbrain.
TS23: hypothalamus	Present		3E
TS23: skeleton	Weak		3E
TS23: thyroid gland	Present		3E

Structure	Level	Pattern	Image	Note
TS23: 4th ventricle	Present	Regionally restricted	3F	Expression is in the periventricular region.
TS23: hypothalamus	Present		3F
TS23: midbrain	Present	Regionally restricted	3F	Expression is in the ventral midbrain.

Structure	Level	Pattern	Image	Note
TS23: nose	Weak	Regionally restricted	3G	Expression is cartilage surrounding the nasal passages.

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present		3H

Structure	Level	Pattern	Image	Note
TS26: esophagus	Present
TS26: hypothalamus	Present
TS26: intestine	Present
TS26: liver	Present
TS26: lung	Present
TS26: pancreas	Present
TS26: skeleton	Present
TS26: stomach	Present
TS26: thyroid gland	Present
TS26: tongue epithelium	Present	Regionally restricted		Expression is in the dorsal epithelium.
TS26: spinal cord floor plate	Present	Regionally restricted		Expression is at the level of the fore- and hindlimb.
TS26: midbrain	Present	Regionally restricted		Expression is in the ventral midbrain.
TS26: 4th ventricle	Present	Regionally restricted		Expression is in the periventricular region.
TS26: nose	Present	Regionally restricted		Expression is cartilage surrounding the nasal passages.
TS26: bladder	Absent
TS26: submandibular gland	Absent

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Absent		4B

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Absent		4D

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Present		4F
TS28: midbrain	Present	Regionally restricted	4F	Expression is motor neurons in the ventral midbrain.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Absent		4H

Structure	Level	Pattern	Image	Note
TS28: taste bud	Absent		5B
TS28: tongue	Absent		5B
TS28: tongue muscle	Absent		5B

Structure	Level	Pattern	Image	Note
TS28: salivary gland	Present	Regionally restricted	5D	Expression is in serous glands and is absent in mucous glands.

Structure	Level	Pattern	Image	Note
TS28: tongue epithelium	Absent		5F	Absent in the ventral epithelium.

Structure	Level	Pattern	Image	Note
TS28: submandibular gland	Absent		5H

Structure	Level	Pattern	Image	Note
TS28: parotid gland	Absent		5J

Structure	Level	Pattern	Image	Note
TS28: esophagus	Absent		6B

Structure	Level	Pattern	Image	Note
TS28: stomach	Present	Regionally restricted	6D	Expression is in the glandular portion.

Structure	Level	Pattern	Image	Note
TS28: small intestine	Present	Regionally restricted	6F	Expression is in the crypts of Lieberkuhn.

Structure	Level	Pattern	Image	Note
TS28: large intestine	Present	Regionally restricted	6H	Expression is in the intestinal glands.

Structure	Level	Pattern	Image	Note
TS28: gallbladder epithelium	Present		7B	Expression is in simple columnar epithelium.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Regionally restricted	7D	Expression is in hepatocytes and bile ducts.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Present	Regionally restricted	7F
TS28: pancreatic acinus	Present	Regionally restricted	7F
TS28: pancreatic duct	Present	Regionally restricted	7F

Structure	Level	Pattern	Image	Note
TS28: olfactory epithelium	Weak		8B

Structure	Level	Pattern	Image	Note
TS28: pharynx	Present	Regionally restricted	8D	Expression is in the mucous glands.

Structure	Level	Pattern	Image	Note
TS28: trachea	Present	Regionally restricted	8F	Scattered or weak staining was seen in proximal trachea in the epithelium but not in the mucous glands.

Structure	Level	Pattern	Image	Note
TS28: lung	Present	Regionally restricted	8H	Expression is in epithelial cells in both conducting airways and in alveolar type II cells.

Structure	Level	Pattern	Image	Note
TS28: thyroid gland	Strong	Regionally restricted	8J	Expression is in a small subset of follicular cells.

Structure	Level	Pattern	Image	Note
TS28: metanephros	Absent		9B

Structure	Level	Pattern	Image	Note
TS28: urinary system	Absent		9D

Structure	Level	Pattern	Image	Note
TS28: bladder	Absent		9F

Structure	Level	Pattern	Image	Note
TS28: male reproductive system	Absent		10B	Absent in the coagulating gland.

Structure	Level	Pattern	Image	Note
TS28: male reproductive system	Absent		10D	Absent in the vesicular gland.

Structure	Level	Pattern	Image	Note
TS28: testis	Absent		10F

Structure	Level	Pattern	Image	Note
TS28: male reproductive system	Absent		10H	Expression is absent in the prostatic urethral wall.

Structure	Level	Pattern	Image	Note
TS28: prostate gland	Absent		10J

Structure	Level	Pattern	Image	Note
TS28: ovary	Absent		11B

Structure	Level	Pattern	Image	Note
TS28: uterus	Very strong	Regionally restricted	11D	Expression is in uterine glands.

Structure	Level	Pattern	Image	Note
TS28: vagina	Absent		11F

Structure	Level	Pattern	Image	Note
TS28: mammary gland	Absent		11H