Hes1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:93571 Rowan S, et al., Transdifferentiation of the retina into pigmented cells in ocular retardation mice defines a new function of the homeodomain gene Chx10. Development. 2004 Oct;131(20):5139-52
Assay type:	RNA in situ
MGI Accession ID:	MGI:3526429
Gene symbol:	Hes1
Gene name:	hes family bHLH transcription factor 1

Probe:	IMAGE clone 5290044
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen E14.5 heterozygote: embryonic day 14.5; Vsx2^or-J/Vsx2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present	Regionally restricted	S1I E14.5 orJ/+	Expression is present in progenitor cells in the outer neuroblastic layer and the periphery of the retina (zone 1 but not zone 2).

Specimen E14.5 homozygote: embryonic day 14.5; Vsx2^or-J/Vsx2^or-J (more

)

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present		S1I E14.5 orJ/orJ	Expression is present in the outer neuroblastic layer.

Specimen E17.5 heterozygote: embryonic day 17.5; Vsx2^or-J/Vsx2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS25: retina outer nuclear layer	Present		S1I E17.5 orJ/+	Expression is present in progenitor cells in the outer neuroblastic layer.
TS25: neural retina	Present	Regionally restricted	S1I E17.5 orJ/+	Expression is present in the periphery of the retina (zone 1 but not zone 2).

Specimen E17.5 homozygote: embryonic day 17.5; Vsx2^or-J/Vsx2^or-J (more

)

Structure	Level	Pattern	Image	Note
TS25: retina outer nuclear layer	Present		S1I E17.5 orJ/orJ
TS25: retina	Present	Regionally restricted	S1I E17.5 orJ/orJ	Expression is present in the center of the eye but is not detected more peripherally in the transdifferentiating region.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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