Gene Expression Data
Assay Details
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Assay
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Reference:
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J:92194
Li Y, et al., Sonic hedgehog signaling regulates Gli3 processing, mesenchymal proliferation, and differentiation during mouse lung organogenesis. Dev Biol. 2004 Jun 1;270(1):214-31
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3526078
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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MEC 13.3
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
6Aa:
embryonic day 15.5
(more  )
Specimen
6Aa:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6Ab:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lung mesenchyme |
Present |
Regionally restricted |
6A b
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Pecam1-positive cells in the interstitial mesenchyme appear to be sparse.
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Specimen
6Ac:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3H/HeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lung mesenchyme |
Present |
Regionally restricted |
6A c
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Pecam1-positive cells in the interstitial mesenchyme are enhanced compared with Shh -/- lungs.
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Specimen
6Ad:
embryonic day 15.5
(more )
Specimen
6Ad:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lung vascular element |
Present |
Uniform |
6A d
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Uniform distribution of Pecam1-positive endothelia from major pulmonary endothelia; not significantly different in wild-type and mutant lungs.
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Specimen
6Ae:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lung vascular element |
Present |
Uniform |
6A e
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Uniform distribution of Pecam1-positive endothelia from major pulmonary endothelia; not significantly different in wild-type and mutant lungs.
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Specimen
6Af:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3H/HeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lung vascular element |
Present |
Uniform |
6A f
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Uniform distribution of Pecam1-positive endothelia from major pulmonary endothelia; not significantly different in wild-type and mutant lungs.
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Analysis Tools
