Gene Expression Data
Assay Details
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Assay
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Reference:
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J:93571
Rowan S, et al., Transdifferentiation of the retina into pigmented cells in ocular retardation mice defines a new function of the homeodomain gene Chx10. Development. 2004 Oct;131(20):5139-52
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3525849
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Gene symbol:
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Mitf
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Gene name:
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melanogenesis associated transcription factor
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Probe:
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Mitf cDNA4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
E14.5 heterozygote:
embryonic day 14.5; Vsx2or-J/Vsx2 +
(more  )
Specimen
E14.5 heterozygote:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6 * FVB/N * SJL
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Mutant Allele(s):
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Vsx2or-J/Vsx2+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
E14.5 homozygote:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6 * FVB/N * SJL
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Mutant Allele(s):
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Vsx2or-J/Vsx2or-J
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
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3F E14.5 orJ/orJ
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There is ectopic expression in the retina and lessened expression in the center.
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Specimen
E17.5 heterozygote:
embryonic day 17.5; Vsx2or-J/Vsx2 +
(more )
Specimen
E17.5 heterozygote:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * FVB/N * SJL
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Mutant Allele(s):
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Vsx2or-J/Vsx2+
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: pigmented retinal epithelium |
Present |
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3F E17.5 orJ/+
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Expression is present throughout the retinal pigmented epithelium (RPE) and at higher levels in the peripheral RPE in zones 2, 3, and 4. There is also expression in migrating melanoblasts.
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Specimen
E17.5 homozygote:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6 * FVB/N * SJL
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Mutant Allele(s):
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Vsx2or-J/Vsx2or-J
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Analysis Tools