Gpc4 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:83696 Ford-Perriss M, et al., Localisation of specific heparan sulfate proteoglycans during the proliferative phase of brain development. Dev Dyn. 2003 Jun;227(2):170-84
Assay type:	Western blot
MGI Accession ID:	MGI:3522632
Gene symbol:	Gpc4
Gene name:	glypican 4

Antibody:	anti-peptide-1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Results

	Sample Information		Bands						Other Sample Information
Lane	Age	Structure	200 kDa (b)	123 kDa	74 kDa	67 kDa (d)	62 kDa	36 kDa (e)	Genetic Background	Mutant Allele(s)	Sex	Note
Anti-pep1 (-)	E10.0 (a)	TS16: embryo	Present	Trace (c)	Trace (c)	Absent	Present	Present	Not Specified		Not Specified
Anti-pep1 (+)	E10.0 (a)	TS16: embryo	Absent	Trace (c)	Trace (c)	Present	Present	Present	Not Specified		Not Specified	Sample was treated with heparitinase I and III.
3G10 (-)	Lane not used in this assay
3G10 (+)	Lane not used in this assay
M	Mol. Wt. Marker Lane

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors describe this band as a high moleuclar weight smear, typical of HSPGs.
(c) Minor bands; likely to represent degraded forms.
(d) Authors state that this is presumably the full length core protein.
(e) Thought to arise from a proteolytic cleavage site in the middle of the core protein.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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