Gene Expression Data
Assay Details
Assay
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Reference:
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J:83696
Ford-Perriss M, et al., Localisation of specific heparan sulfate proteoglycans during the proliferative phase of brain development. Dev Dyn. 2003 Jun;227(2):170-84
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3522619
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Gene symbol:
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Sdc1
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Gene name:
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syndecan 1
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Antibody:
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281-2
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
2A:
embryonic day 10.5
(more )
Specimen
2A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: forebrain |
Strong |
Regionally restricted |
2A
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Strong immunoreactivity througout neuroepithelium of forebrain, midbrain and hindbrain. Authors report all neuroepithelial cells in the neural tube are positive, and stronger staining at the luminal side of the neural tube compared with the basal side.
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TS17: hindbrain |
Strong |
Regionally restricted |
2A
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Strong immunoreactivity througout neuroepithelium of forebrain, midbrain and hindbrain. Authors report all neuroepithelial cells in the neural tube are positive, and stronger staining at the luminal side of the neural tube compared with the basal side.
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TS17: midbrain |
Strong |
Regionally restricted |
2A
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Strong immunoreactivity througout neuroepithelium of forebrain, midbrain and hindbrain. Authors report all neuroepithelial cells in the neural tube are positive, and stronger staining at the luminal side of the neural tube compared with the basal side.
|
Specimen
2A':
embryonic day 10.5
(more )
Specimen
2A':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: telencephalon |
Strong |
Regionally restricted |
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Section through dorsal telencephalon. Rounded mitotic cells at luminal side of neural tube are outlined and intensely stained with immunoreactivity. The ectoderm is also strongly positive.
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Specimen
2B',2B'':
embryonic day 10.5
(more )
Specimen
2B',2B'':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: midbrain |
Present |
Regionally restricted |
2B''
,
2B'
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Expression appears to be restricted to neuroepithelial cells, and downregulated upon differentiation into neurons.
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Specimen
2C',2C'':
embryonic day 10.5
(more )
Specimen
2C',2C'':
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindbrain |
Present |
Regionally restricted |
2C'
,
2C''
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Expression appears to be restricted to neuroepithelial cells, and downregulated upon differentiation into neurons.
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