Gene Expression Data
Assay Details
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Assay
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Reference:
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J:88577
Mu X, et al., Discrete gene sets depend on POU domain transcription factor Brn3b/Brn-3.2/POU4f2 for their expression in the mouse embryonic retina. Development. 2004 Mar;131(6):1197-210
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3522266
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Gene symbol:
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Mstn
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Gene name:
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myostatin
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Probe:
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Gdf8 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
+/-:
embryonic day 14.5; Pou4f2tm3Whk/Pou4f2 +
(more  )
Specimen
+/-:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Pou4f2tm3Whk/Pou4f2+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
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2C GDF8 +/-
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Expression was confined to retinal ganglion cells.
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Specimen
-/-:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Pou4f2tm3Whk/Pou4f2tm3Whk
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Absent |
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2C GDF8 -/-
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Authors state that expression was largely absent in -/- retinas.
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Analysis Tools