Igf1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:86408 Villalpando I, et al., Insulin-like growth factor I (IGF-I) regulates endocrine activity of the embryonic testis in the mouse. J Steroid Biochem Mol Biol. 2003 Aug;86(2):151-8
Assay type:	RT-PCR
MGI Accession ID:	MGI:3513084
Gene symbol:	Igf1
Gene name:	insulin-like growth factor 1

Probe:	Igf1-pG, Igf1-pH
Visualized with:	Ethidium bromide
Assay notes:	These data are quantified in Fig. 2.

Results

Image: 1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	352 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
ST	Mol. Wt. Marker Lane
1	E10.5 (a)	TS17: gonad primordium	Present	Not Specified; total RNA	CD-1		Male
2	Control
3	E11.5 (a)	TS19: gonad	Present	Not Specified; total RNA	CD-1		Male
4	Control
5	E12.5 (a)	TS20: testis	Present (b)	Not Specified; total RNA	CD-1		Male
6	Control
7	E13.5 (a)	TS21: testis	Present (b)	Not Specified; total RNA	CD-1		Male
8	Control
9	E14.5 (a)	TS22: testis	Present	Not Specified; total RNA	CD-1		Male
10	Control
11	E15.5 (a)	TS23: testis	Present (c)	Not Specified; total RNA	CD-1		Male
12	Control
13	E16.5 (a)	TS24: testis	Present	Not Specified; total RNA	CD-1		Male
14	Control
15	E17.5 (a)	TS25: testis	Present (d)	Not Specified; total RNA	CD-1		Male
16	Control

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) This is one of the times with the lowest level of expression.
(c) The highest expression levels are observed at this time point.
(d) Expression levels are similar to those seen at E11.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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