Gene Expression Data
Assay Details
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Assay
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Reference:
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J:89008
Davy A, et al., Ephrin-B1 forward and reverse signaling are required during mouse development. Genes Dev. 2004 Mar 1;18(5):572-83
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3512291
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Gene symbol:
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Efnb1
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Gene name:
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ephrin B1
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Probe:
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Efnb1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2Ba WT:
embryonic day 11.5
(more  )
Specimen
2Ba WT:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2Bb het:
embryonic day 11.5; Efnb1tm1.1Sor/Efnb1 +
(more )
Specimen
2Bb het:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Efnb1tm1.1Sor/Efnb1+
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb |
Present |
Regionally restricted |
2Bb
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Expression is in alternating rays of expressing and non-expressing cells.
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Specimen
2Bc mutant:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Efnb1tm1.1Sor/Efnb1tm1.1Sor
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2Da WT:
embryonic day 10.5
(more )
Specimen
2Da WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2Db mutant:
embryonic day 10.5; Efnb1tm1.1Sor/Efnb1 +
(more )
Specimen
2Db mutant:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Efnb1tm1.1Sor/Efnb1+
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: limb |
Present |
Regionally restricted |
2Db
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Expression is patchy.
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Analysis Tools