Snrpn RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:91108 Kaneda M, et al., Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting. Nature. 2004 Jun 24;429(6994):900-3
Assay type:	RT-PCR
MGI Accession ID:	MGI:3511579
Gene symbol:	Snrpn
Gene name:	small nuclear ribonucleoprotein N

Probe:	Snrpn-pG, Snrpn-pH
Assay notes:	The RT-PCR products from this reaction were subjected to restriction digest to reveal allele-specific expression.

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Size Not Specified (c)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
B x J	E10.5 (a)	TS17: embryo	Absent	Present (d)	Not Specified; total RNA	(C57BL/6 x JF1)F1		Not Specified
J X B	E10.5 (a)	TS17: embryo	Present (d)	Absent	Not Specified; total RNA	(JF1 x C57BL/6)F1		Not Specified
5	E10.5 (a)	TS17: embryo	Present (e)	Present (e)	Not Specified; total RNA	involves: 129S4/SvJae * C57BL/6 * JF1	Dnmt3a^tm3.2Enl/Dnmt3a⁺	Not Specified	embryo's mother was a conditional mutant in the germ line
6	E10.5 (a)	TS17: embryo	Present (e)	Present (e)	Not Specified; total RNA	involves: 129S4/SvJae * C57BL/6 * JF1	Dnmt3a^tm3.2Enl/Dnmt3a⁺	Not Specified	embryo's mother was a conditional mutant in the germ line

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) This band represents expression from the B6 allele.
(c) This band represents expression from the JF1 allele.
(d) This band reflects expression from the maternal allele.
(e) Expression is biallelic in the mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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