Pitx2 RNA in situ Gene Expression Assay - GXD

About Help FAQ

Gene Expression Data
Assay Details

Assay

Reference:	J:92872 Bamforth SD, et al., Cited2 controls left-right patterning and heart development through a Nodal-Pitx2c pathway. Nat Genet. 2004 Dec;36(11):1189-96
Assay type:	RNA in situ
MGI Accession ID:	MGI:3511349
Gene symbol:	Pitx2
Gene name:	paired-like homeodomain transcription factor 2

Probe:	Pitx2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

3e ©

Specimen 3E: embryonic day 8.5 (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS13: mesenchyme derived from head mesoderm	Present		3e
TS13: lateral plate mesoderm	Present	Regionally restricted	3e	Expression is on the left side.

3f ©

Specimen 3F: embryonic day 8.5 (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS13: mesenchyme derived from head mesoderm	Present		3f
TS13: lateral plate mesoderm	Present	Regionally restricted	3f	Expression is on the left side.

3g ©

Specimen 3G: embryonic day 8.5; Cited2^tm1Bha/Cited2^tm1Bha (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS13: mesenchyme derived from head mesoderm	Present		3g
TS13: lateral plate mesoderm	Absent		3g

3h ©

Specimen 3H: embryonic day 8.5; Cited2^tm1Bha/Cited2^tm1Bha (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS13: mesenchyme derived from head mesoderm	Present		3h
TS13: lateral plate mesoderm	Absent		3h

3i ©

Specimen 3I: embryonic day 15.5 (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS23: left atrium	Present		3i
TS23: heart right ventricle	Present	Regionally restricted	3i
TS23: heart	Present	Regionally restricted	3i	Expression is in the outflow tract.

3j ©

Specimen 3J: embryonic day 15.5 (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS23: left atrium	Present		3j
TS23: heart right ventricle	Present	Regionally restricted	3j	Expression is on the ventral surface.
TS23: heart	Present	Regionally restricted	3j	Expression is in the outflow tract.

3k ©

Specimen 3K: embryonic day 15.5; Cited2^tm1Bha/Cited2^tm1Bha (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS23: heart	Absent		3k
TS23: heart right ventricle	Absent		3k
TS23: left atrium	Absent		3k

3l ©

Specimen 3L: embryonic day 15.5; Cited2^tm1Bha/Cited2^tm1Bha (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS23: heart	Absent		3l
TS23: heart right ventricle	Absent		3l
TS23: left atrium	Absent		3l

3m ©

Specimen 3M: embryonic day 8.5 (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS13: mesenchyme derived from head mesoderm	Present		3m
TS13: lateral plate mesoderm	Present	Regionally restricted	3m	Expression is on the left.

3n ©

Specimen 3N: embryonic day 8.5 (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS13: mesenchyme derived from head mesoderm	Present		3n
TS13: lateral plate mesoderm	Present	Regionally restricted	3n	Expression is on the left.

3o ©

Specimen 3O: embryonic day 8.5; Cited2^tm1Bha/Cited2^tm1Bha (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS13: mesenchyme derived from head mesoderm	Present		3o	Expression in this mutant is the same as in wild-type embryos.
TS13: lateral plate mesoderm	Present	Regionally restricted	3o	Expression is on the left-hand side. Expression in this mutant is the same as in wild-type embryos.

3p ©

Specimen 3P: embryonic day 8.5; Cited2^tm1Bha/Cited2^tm1Bha (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS13: mesenchyme derived from head mesoderm	Present		3p	Expression in this mutant is the same as in wild-type embryos.
TS13: lateral plate mesoderm	Present	Regionally restricted	3p	Expression is on the left-hand side. Expression in this mutant is the same as in wild-type embryos.

3q ©

Specimen 3Q: embryonic day 15.5 (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS23: left atrium	Present		3q
TS23: heart right ventricle	Present	Regionally restricted	3q	Expression is on the ventral surface.
TS23: heart	Present	Regionally restricted	3q	Expression is in the outflow tract.

3r ©

Specimen 3R: embryonic day 15.5 (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS23: left atrium	Present		3r
TS23: heart right ventricle	Present	Regionally restricted	3r	Expression is on the ventral surface.
TS23: heart	Present	Regionally restricted	3r	Expression is in the outflow tract.

3s ©

Specimen 3S: embryonic day 15.5; Cited2^tm1Bha/Cited2^tm1Bha (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS23: left atrium	Present		3s	This expression was seen in one out of three mutant embryos.
TS23: heart right ventricle	Weak	Regionally restricted	3s	The expression is on the ventral surface. This expression was seen in one out of three mutant embryos.
TS23: heart	Weak	Regionally restricted	3s	The expression is in the outflow tract. This expression was seen in one out of three mutant embryos.

3t ©

Specimen 3T: embryonic day 15.5; Cited2^tm1Bha/Cited2^tm1Bha (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS23: left atrium	Present		3t	This expression was seen in one out of three mutant embryos.
TS23: heart right ventricle	Weak	Regionally restricted	3t	The expression is on the ventral surface. This expression was seen in one out of three mutant embryos.
TS23: heart	Weak	Regionally restricted	3t	The expression is in the outflow tract. This expression was seen in one out of three mutant embryos.

Specimen Not shown: embryonic day 15.5; Cited2^tm1Bha/Cited2^tm1Bha (more

)
Note: Left lateral and ventral views.

Structure	Level	Pattern	Image	Note
TS23: heart	Present	Regionally restricted		Normal expression was seen in the outflow tract in 2/3 embryos.
TS23: left atrium	Present			Normal expression is seen in 2/3 embryos.
TS23: heart right ventricle	Present	Regionally restricted		Normal expression along the ventral surface was seen in 2/3 embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/18/2025 MGI 6.24