Gene Expression Data
Assay Details
Assay
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Reference:
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J:21125
Lee CH, et al., Normal onset, but prolonged expression, of Sry gene in the B6.YDOM sex-reversed mouse gonad. Dev Biol. 1994 Oct;165(2):442-52
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:3497938
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Gene symbol:
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Sry
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Gene name:
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sex determining region of Chr Y
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Probe:
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Sry-pI, Sry-pJ
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Probe preparation:
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DNA
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Visualized with:
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Ethidium bromide
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Assay notes:
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Analyses were performed in C57BL/6J mice which contained the Y chromosome of M. m. domesticus. A large number of gonads were examined and a summary of RT-PCR analyses in B6.YDom is shown as a percentage of positive results in Figure 4.
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Results
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Mol. Wt. Marker Lane |
E10.5
(a)
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TS17: gonad primordium
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Present
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Not Specified; total RNA |
involves: C57BL/6J * M. m. domesticus |
| Not Specified |
Morphologically undifferentiated gonad.
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Control |
E11.5
(a)
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TS19: gonad
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Present
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Not Specified; total RNA |
involves: C57BL/6J * M. m. domesticus |
| Not Specified |
Morphologically undifferentiated gonad.
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Control |
E12.5
(a)
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TS20: gonad
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Present
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Not Specified; total RNA |
involves: C57BL/6J * M. m. domesticus |
| Male |
Morphologically undifferentiated gonad.
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Control |
Mol. Wt. Marker Lane |
E13.5
(a)
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TS21: gonad
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Present
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Not Specified; total RNA |
involves: C57BL/6J * M. m. domesticus |
| Not Specified |
Ovary (sex-reversed).
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Control |
E14.5
(a)
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TS22: gonad
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Present
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Not Specified; total RNA |
involves: C57BL/6J * M. m. domesticus |
| Not Specified |
Ovary (sex-reversed).
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Control |
E15.5
(a)
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TS23: gonad
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Absent
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Not Specified; total RNA |
involves: C57BL/6J * M. m. domesticus |
| Not Specified |
Ovary (sex-reversed).
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Control |
E16.5
(a)
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TS24: gonad
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Absent
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Not Specified; total RNA |
involves: C57BL/6J * M. m. domesticus |
| Not Specified |
Ovary (sex-reversed).
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Control |
E18.5
(a)
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TS26: gonad
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Absent
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Not Specified; total RNA |
involves: C57BL/6J * M. m. domesticus |
| Not Specified |
Ovary (sex-reversed).
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Control |
Mol. Wt. Marker Lane |
E13.5
(a)
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TS21: gonad
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Present
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Not Specified; total RNA |
involves: C57BL/6J * M. m. domesticus |
| Not Specified |
Ovotestis.
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Control |
E14.5
(a)
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TS22: gonad
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Weak
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Not Specified; total RNA |
involves: C57BL/6J * M. m. domesticus |
| Not Specified |
Ovotestis.
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Control |
E15.5
(a)
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TS23: gonad
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Absent
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Not Specified; total RNA |
involves: C57BL/6J * M. m. domesticus |
| Not Specified |
Ovotestis.
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Control |
E16.5
(a)
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TS24: gonad
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Absent
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Not Specified; total RNA |
involves: C57BL/6J * M. m. domesticus |
| Not Specified |
Ovotestis.
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Control |
E18.5
(a)
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TS26: gonad
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Absent
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Not Specified; total RNA |
involves: C57BL/6J * M. m. domesticus |
| Not Specified |
Ovotestis.
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Control |
Notes:
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