Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:91012
Kurokawa D, et al., Regulation of Otx2 expression and its functions in mouse epiblast and anterior neuroectoderm. Development. 2004 Jul;131(14):3307-17
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3056072
|
|
Gene symbol:
|
Gbx2
|
|
Gene name:
|
gastrulation brain homeobox 2
|
|
Probe:
|
Gbx2 cDNA10
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
6Bg:
embryonic day 8.5
(more  )
Specimen
6Bg:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6Bh:
(close )
|
Genetic Background:
|
involves: C57BL/6NCrlj * CBA/JNCrlj
|
|
Mutant Allele(s):
|
Otx2tm2.1Imat/Otx2tm7Sia
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Present |
Regionally restricted |
6B h
|
The negative region is reduced in this mutant.
|
Specimen
6Bi:
embryonic day 9.5
(more )
Specimen
6Bi:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6Bj:
(close )
|
Genetic Background:
|
involves: C57BL/6NCrlj * CBA/JNCrlj
|
|
Mutant Allele(s):
|
Otx2tm2.1Imat/Otx2tm7Sia
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6Ce:
embryonic day 11.5
(more )
Specimen
6Ce:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
6C e
|
Expression is in the dorsal thalamus.
|
Specimen
6Cf:
(close )
|
Genetic Background:
|
involves: C57BL/6NCrlj * CBA/JNCrlj
|
|
Mutant Allele(s):
|
Emx2tm1Sia/Emx2tm1Sia
Otx2tm7Sia/Otx2tm7Sia
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Absent |
|
6C f
|
In this mutant there is no expression in the dorsal thalamus. This indicates that the diencephalon is missing.
|
|
Analysis Tools