Slit3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:86149 Liu J, et al., Congenital diaphragmatic hernia, kidney agenesis and cardiac defects associated with Slit3-deficiency in mice. Mech Dev. 2003 Sep;120(9):1059-70
Assay type:	RT-PCR
MGI Accession ID:	MGI:3055376
Gene symbol:	Slit3
Gene name:	slit guidance ligand 3

Probe:	5' primer pair
Assay notes:	27 cycles

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	300 bp (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
+/+	P newborn	TS27: mouse	Present	1 µg; total RNA	Not Specified		Not Specified
+/+	P newborn	TS27: mouse	Present	1 µg; total RNA	Not Specified		Not Specified
-/-	P newborn	TS27: mouse	Absent	1 µg; total RNA	involves: 129S5/SvEvBrd * C57BL/6	Slit3^{Gt(OST106158)Lex}/Slit3^{Gt(OST106158)Lex}	Not Specified
-/-	P newborn	TS27: mouse	Absent	1 µg; total RNA	involves: 129S5/SvEvBrd * C57BL/6	Slit3^{Gt(OST106158)Lex}/Slit3^{Gt(OST106158)Lex}	Not Specified
-/-	P newborn	TS27: mouse	Absent	1 µg; total RNA	involves: 129S5/SvEvBrd * C57BL/6	Slit3^{Gt(OST106158)Lex}/Slit3^{Gt(OST106158)Lex}	Not Specified

Notes:
(a) The authors state that no fragments were obtained from the homozygous animals after 27 cycles, but were seen after 35 cycles of amplification (see assay MGI:3055386) and suggest that there was a remnant expression of Slit3 mRNA in the homozygous animals.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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