Gata4 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:91025 Parlakian A, et al., Targeted inactivation of serum response factor in the developing heart results in myocardial defects and embryonic lethality. Mol Cell Biol. 2004 Jun;24(12):5281-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:3054932
Gene symbol:	Gata4
Gene name:	GATA binding protein 4

Probe:	Gata4-pG, Gata4-pH
Visualized with:	Ethidium bromide

Results

Image: 6_gata4

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
+ S^f/S^f	E9.5 (a)	TS15: heart	Present (b)	1 µg; total RNA	involves: 129/Sv * C57BL/6	Srf^tm1Zli/Srf^tm1Zli, Tg(Myh7-cre)1Zli/0	Not Specified	Conditional mutant.
- S^f/S^f	E9.5 (a)	TS15: heart	Present	1 µg; total RNA	involves: 129/Sv * C57BL/6	Srf^tm1Zli/Srf^tm1Zli	Not Specified
+ S^f/S^f	E9.5 (a)	TS15: head TS15: trunk	Present (c)	1 µg; total RNA	involves: 129/Sv * C57BL/6	Srf^tm1Zli/Srf^tm1Zli, Tg(Myh7-cre)1Zli/0	Not Specified	Conditional mutant. This upper body preparation includes the head, cervical somites, upper aortae, pharyngeal arches, and limb buds.
- S^f/S^f	E9.5 (a)	TS15: head TS15: trunk	Present	1 µg; total RNA	involves: 129/Sv * C57BL/6	Srf^tm1Zli/Srf^tm1Zli	Not Specified	This upper body preparation includes the head, cervical somites, upper aortae, pharyngeal arches, and limb buds.

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression levels are markedly reduced in the mutant.
(c) Expressions levels do not differ from those seen in wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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