Gfap Northern blot Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:64290 McCall MA, et al., Targeted deletion in astrocyte intermediate filament (Gfap) alters neuronal physiology. Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6361-6
Assay type:	Northern blot
MGI Accession ID:	MGI:3052493
Gene symbol:	Gfap
Gene name:	glial fibrillary acidic protein

Probe:	pGFAP-G1
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography

Results

Image: 2_GFAP ©

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
-/-	P adult	TS28: brain	Absent	10 µg; total RNA	either: (involves: 129S7/SvEvBrd * 129X1/SvJ) or (involves: 129S7/SvEvBrd * C57BL/6)	Gfap^tm1Mes/Gfap^tm1Mes	Not Specified
-/-	P adult	TS28: brain	Absent	10 µg; total RNA	either: (involves: 129S7/SvEvBrd * 129X1/SvJ) or (involves: 129S7/SvEvBrd * C57BL/6)	Gfap^tm1Mes/Gfap^tm1Mes	Not Specified
+/-	P adult	TS28: brain	Present (a)	10 µg; total RNA	either: (involves: 129S7/SvEvBrd * 129/Sv) or (involves: 129S7/SvEvBrd * C57BL/6)	Gfap^tm1Mes/Gfap⁺	Not Specified
+/+	P adult	TS28: brain	Present	10 µg; total RNA	Not Specified		Not Specified
+/+	P adult	TS28: brain	Present	10 µg; total RNA	Not Specified		Not Specified
+/+	E14.0 (b)	TS22: embryo	Absent	10 µg; total RNA	Not Specified		Not Specified

Notes:
(a) Expression levels are reduced in the heterozygote.
(b) Age of embryo at noon of plug day not specified in reference.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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