Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75934
Zelzer E, et al., Skeletal defects in VEGF(120/120) mice reveal multiple roles for VEGF in skeletogenesis. Development. 2002 Apr;129(8):1893-904
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3047233
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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Anti-CD31
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Results
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Specimen
3A:
embryonic day 14.5
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel |
Present |
Regionally restricted |
3A
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Expression was detected in the vessels surrounding the tibia.
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Vegfatm1Pec/Vegfatm1Pec
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel |
Present |
Regionally restricted |
3B
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Expression was detected in vessels, but unlike wild type, they remain far from the surface of the tibia and fibula.
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Specimen
3C:
embryonic day 15.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: blood vessel |
Present |
Regionally restricted |
3C
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Expression was detected in vessels that had penetrated the primary ossification center of the tibia.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Vegfatm1Pec/Vegfatm1Pec
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: blood vessel |
Present |
Regionally restricted |
3D
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Expression was detected in the vessels surrounding the tibia and fibula, but they had not yet invaded the hypertrophic cartilage.
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Specimen
3E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Vegfatm1Pec/Vegfatm1Pec
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: blood vessel |
Present |
Regionally restricted |
3E
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Expression was detected in vessels invading the diaphysis.
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Specimen
3F:
embryonic day 16.5
(more )
Specimen
3F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: blood vessel |
Present |
Regionally restricted |
3F
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Expression was detected in the vessels surrounding the calvarial bones.
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Specimen
3G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Vegfatm1Pec/Vegfatm1Pec
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: blood vessel |
Present |
Regionally restricted |
3G
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Expression was detected in vessels, but they were fewer and larger in diameter compared to wild type.
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Analysis Tools