Gene Expression Data
Assay Details
Assay
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Reference:
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J:34073
Adams RH, et al., A novel class of murine semaphorins with homology to thrombospondin is differentially expressed during early embryogenesis. Mech Dev. 1996 Jun;57(1):33-45
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3044160
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Gene symbol:
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Sema5a
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Gene name:
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sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
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Probe:
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SemF cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
8E:
embryonic day 11.0
(more )
Specimen
8E:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 11.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS18: forelimb bud |
Present |
Regionally restricted |
8E
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Expresion is in a stripe at the base of the limb.
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Specimen
8F:
embryonic day 12.0
(more )
Specimen
8F:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 12.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: head |
Strong |
Regionally restricted |
8F
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Expression is in the branchial arches.
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Specimen
8G:
embryonic day 12.0
(more )
Specimen
8G:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 12.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: head |
Strong |
Regionally restricted |
8G
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Expression is in the branchial arches.
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Specimen
8I:
embryonic day 13.0
(more )
Specimen
8I:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 13.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
8J:
embryonic day 13.0
(more )
Specimen
8J:
(close )
Genetic Background:
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NMRI
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Age:
|
embryonic day 13.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Not Shown:
embryonic day 15.5
(more )
Specimen
Not Shown:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS23: limb |
Weak |
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