Htt RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:90356 Dixon KT, et al., Mouse Huntington's disease homolog mRNA levels: variation and allele effects. Gene Expr. 2004;11(5-6):221-31
Assay type:	RT-PCR
MGI Accession ID:	MGI:3043480
Gene symbol:	Htt
Gene name:	huntingtin

Probe:	Hdh-pE, Hdh-pF
Probe preparation:	DNA
Assay notes:	Assay was done using the qRT-PCR system from Applied Biosystems. Data in Figure 3 shows variation in tissue Hhd levels between 6 WT and 6 Hdh+/- mice. Figure 4 shows the individual tissue levels for each mouse and discusses the effect of high Hdh levels in one tissue on Hdh levels in other tissues.

Results

Image: 3

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
T +/+	P adult	TS28: testis	Strong	5 µg; total RNA	C57BL/6J		Male
B +/+	P adult	TS28: brain	Present	5 µg; total RNA	C57BL/6J		Male
K +/+	P adult	TS28: metanephros	Present	5 µg; total RNA	C57BL/6J		Male
S +/+	P adult	TS28: spleen	Present	5 µg; total RNA	C57BL/6J		Male
H +/+	P adult	TS28: heart	Weak	5 µg; total RNA	C57BL/6J		Male
L +/+	P adult	TS28: liver	Weak	5 µg; total RNA	C57BL/6J		Male
P +/+	P adult	TS28: pancreas	Weak	5 µg; total RNA	C57BL/6J		Male
M +/+	P adult	TS28: skeletal muscle	Weak	5 µg; total RNA	C57BL/6J		Male
T +/-	P adult	TS28: testis	Present	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm3Detl/Htt⁺	Male
B +/-	P adult	TS28: brain	Present	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm3Detl/Htt⁺	Male
K +/-	P adult	TS28: metanephros	Present	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm3Detl/Htt⁺	Male
S +/-	P adult	TS28: spleen	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm3Detl/Htt⁺	Male
H +/-	P adult	TS28: heart	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm3Detl/Htt⁺	Male
L +/-	P adult	TS28: liver	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm3Detl/Htt⁺	Male
P +/-	P adult	TS28: pancreas	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm3Detl/Htt⁺	Male
M +/-	P adult	TS28: skeletal muscle	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm3Detl/Htt⁺	Male

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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