Probe:	Fgfr2IIIb cDNA3
Probe preparation:	Antisense RNA
Visualized with:	Autoradiography
Assay notes:	The probe used in this assay was specific for the KGFR isoform.

Structure	Level	Pattern	Image	Note
TS8: extraembryonic component	Weak		2C/D
TS8: embryo	Absent		2C/D	Authors believe that most of the embryo fails to express.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	2E/F	Expression was present at the junction of the embryonic and extraembryonic areas and includes all three germinal layers.
TS10: extraembryonic component	Present	Regionally restricted	2E/F	Expression was present at the junction of the embryonic and extraembryonic areas and includes all three germinal layers.

Structure	Level	Pattern	Image	Note
TS11: extraembryonic component	Present		2G/H	Expression was present in all three germ layers.
TS11: embryo	Weak		2G/H	Expression was diffusely distributed throughout the embryo, including all three germ layers.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present		4A/B
TS13: neural ectoderm	Present		4A/B
TS13: trunk somite	Present		4A/B

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Strong		4E/F
TS17: future spinal cord	Present		4E/F
TS17: nephrogenic cord	Present		4E/F
TS17: hindgut	Present	Regionally restricted	4E/F	Expression was present in the hindgut endoderm.

Structure	Level	Pattern	Image	Note
TS20: skin	Strong			There is a continuous contour of expression around the embryo.
TS20: nasal cavity epithelium	Present
TS20: oral epithelium	Present
TS20: pharynx epithelium	Present

Structure	Level	Pattern	Image	Note
TS22: vault of skull	Present			Expression is present in the skull.

Structure	Level	Pattern	Image	Note
TS22: skin	Strong		5A/B	There is a continuous contour of expression around the embryo.
TS22: rib cartilage condensation	Present		5A/B
TS22: vault of skull	Present		5A/B
TS22: vertebral cartilage condensation	Present		5A/B
TS22: pelvic girdle skeleton	Present		5A/B	Expression is present in the ilium bone.
TS22: limb	Present		5A/B	Expression is present in the long bones of the limb.
TS22: facial bone primordium	Present		5A/B
TS22: naris	Present		5A/B	Expression is present in the bones of the nasal cavity.
TS22: nasal cavity epithelium	Present		5A/B
TS22: oral epithelium	Present		5A/B
TS22: pharynx epithelium	Present		5A/B
TS22: gut	Present	Regionally restricted	5A/B	Expression is present in the endothelium.
TS22: stomach	Present	Regionally restricted	5A/B	Expression is present in the endothelium.
TS22: pancreas	Present	Regionally restricted	5A/B	Expression is present in the epithelium.
TS22: liver	Present		5A/B	There is diffuse expression in the liver.
TS22: collecting duct	Present		5A/B
TS22: remnant of Rathke's pouch	Present		5A/B
TS22: thymus primordium	Present		5A/B

Structure	Level	Pattern	Image	Note
TS22: epidermis	Present		5D/E	Expression is present in the periderm of skin from the thoracic region.
TS22: dermis	Absent		5D/E	There is no expression in the dermis of skin from the thoracic region.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present		5G/H	Expression is present in the alveolar epithelium.

Structure	Level	Pattern	Image	Note
TS22: ossicle	Present		5J/K	Expression is present in the auditory ossicles.
TS22: inner ear	Present	Regionally restricted	5J/K	Expression is present in the bony part of the inner ear.
TS22: cochlear duct epithelium	Present		5J/K

Structure	Level	Pattern	Image	Note
TS22: lower jaw incisor enamel organ	Present	Regionally restricted	5M/N	Expression is present in the superficial portion of the organ.
TS22: salivary gland	Present	Regionally restricted	5M/N	Expression is present close to the lumen.

Structure	Level	Pattern	Image	Note
TS22: cornea epithelium	Present		5P/R
TS22: facial bone primordium	Present		5P/R
TS22: lens epithelium	Present		5P/R
TS22: naris	Present	Regionally restricted	5P/R	Expression is present in the bones of the nasal cavity.

Structure	Level	Pattern	Image	Note
TS22: nasal cavity epithelium	Present		5T/U
TS22: vibrissa	Present	Regionally restricted	5T/U	Expression is present in the root sheath.
TS22: epidermis	Present		5T/U	Expression is present in the periderm of skin from the nasal areas.
TS22: dermis	Absent		5T/U	There is no expression in the dermis of skin from the nasal areas.
TS22: facial bone primordium	Present		5T/U
TS22: naris	Present	Regionally restricted	5T/U	Expression is present the bones of the nasal cavity.

Structure	Level	Pattern	Image	Note
TS22: humerus cartilage condensation	Present	Regionally restricted	5W/X	Expression is present in the long bones of the limb.
TS22: radius cartilage condensation	Present	Regionally restricted	5W/X	Expression is present in the long bones of the limb.
TS22: ulna cartilage condensation	Present	Regionally restricted	5W/X	Expression is present in the long bones of the limb.
TS22: rib cartilage condensation	Present		5W/X
TS22: gut	Present	Regionally restricted	5W/X	Expression is present in the endothelium of the gut.
TS22: stomach	Present	Regionally restricted	5W/X	Expression is present in the endothelium.