Probe:

Spry2 probe1

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Uniform	2B
TS9: extraembryonic component cavity	Present	Uniform	2B
TS9: extraembryonic ectoderm	Present	Uniform	2B
TS9: extraembryonic endoderm	Present	Uniform	2B
TS9: mural trophectoderm	Present	Uniform	2B
TS9: ectoplacental cone	Absent		2B
TS9: primitive streak	Present	Graded	2B

Structure	Level	Pattern	Image	Note
TS9: extraembryonic visceral endoderm	Present		2E
TS9: embryo	Present		2E	Expression is in the epiblast.
TS9: embryo mesoderm	Present	Regionally restricted	2E	Expression is in the mesodermal wings.
TS9: primitive streak	Present	Graded	2E	Expression is stronger in the anterior part.

Structure	Level	Pattern	Image	Note
TS9: primitive streak	Present	Graded	2H	Expression is stronger in the anterior part.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present		2K
TS12: embryo mesenchyme	Present	Regionally restricted	2K	Expression is in the anterior mesoderm.

Structure	Level	Pattern	Image	Note
TS16: future forebrain	Present		2N
TS16: limb	Present		2N
TS16: tail	Present		2N
TS16: trunk somite	Present		2N
TS16: future hindbrain	Present	Regionally restricted	2N	Expression spans the isthmus, the posterior midbrain and anterior hindbrain.
TS16: future midbrain	Present	Regionally restricted	2N	Expression spans the isthmus, the posterior midbrain and anterior hindbrain.
TS16: telencephalon	Present	Regionally restricted	2N	Expression is in the commissural plate.
TS16: branchial pouch	Present	Regionally restricted	2N	Expression is in the extreme lateral region.
TS16: 1st branchial arch mesenchyme	Present		2N
TS16: 2nd branchial arch mesenchyme	Present		2N
TS16: 3rd branchial arch mesenchyme	Present		2N
TS16: branchial arch ectoderm	Present		2N

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	3B	Expression is in the mid/hindbrain boundary.
TS20: midbrain	Present	Regionally restricted	3B	Expression is in the mid/hindbrain boundary.
TS20: limb	Present		3B
TS20: tail	Present		3B
TS20: head	Present	Regionally restricted	3B	Expression is in the branchial arches.
TS20: telencephalon	Present	Regionally restricted	3B	Expression is in the commissural plate.
TS20: cerebral cortex	Present		3B
TS20: dorsal root ganglion	Present		3B
TS20: lung	Present		3B
TS20: metanephros	Present		3B
TS20: stomach	Present		3B
TS20: tail somite	Present		3B

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present		3E
TS20: oral epithelium	Present		3E
TS20: telencephalon	Present	Regionally restricted	3E	Expression is in the commissural plate and in the medial septal area.
TS20: forebrain	Present	Regionally restricted	3E	Expression is in the hippocampus.
TS20: tongue	Present		3E
TS20: eye	Present	Regionally restricted	3E	Expression is in the optic cup.
TS20: lens vesicle	Present		3E
TS20: head mesenchyme	Present	Regionally restricted	3E	Expression is in the mesenchyme of the first branchial arch.

Structure	Level	Pattern	Image	Note
TS20: neurohypophysis infundibulum	Present		3H
TS20: remnant of Rathke's pouch	Present		3H
TS20: head mesenchyme	Present	Regionally restricted	3H	Expression is in the mesenchyme of the first branchial arch.
TS20: trigeminal ganglion	Strong		3H

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present		3K	Expression is in the mid/hindbrain boundary and in the dorsal regions of the myelencephalon.
TS20: midbrain	Present		3K	Expression is in the mid/hindbrain boundary and in the dorsal regions of the myelencephalon.
TS20: cerebellum primordium	Present		3K

Structure	Level	Pattern	Image	Note
TS23: hindbrain	Present	Regionally restricted	4B	Expression is in the mid/hindbrain boundary.
TS23: midbrain	Present	Regionally restricted	4B	Expression is in the mid/hindbrain boundary.
TS23: submandibular gland primordium	Strong		4B
TS23: duodenum caudal part epithelium	Present		4B
TS23: duodenum rostral part epithelium	Present		4B