Numb Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:58382 Dho SE, et al., Characterization of four mammalian numb protein isoforms. Identification of cytoplasmic and membrane-associated variants of the phosphotyrosine binding domain. J Biol Chem. 1999 Nov 12;274(46):33097-104
Assay type:	Western blot
MGI Accession ID:	MGI:3040077
Gene symbol:	Numb
Gene name:	NUMB endocytic adaptor protein

Antibody:	anti-PRRi
Assay notes:	Samples were immunoprecipitated using the anti-Nb-C antibody (MGI:3040050) prior to immunoblotting.

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	72 kDa (a)	71 kDa (b)	Genetic Background	Mutant Allele(s)	Sex
brain	P adult	TS28: brain	Not Specified	Trace	Not Specified		Not Specified
lung	P adult	TS28: lung	Not Specified	Trace	Not Specified		Not Specified
testis	P adult	TS28: testis	Not Specified	Present	Not Specified		Male
thymus	P adult	TS28: thymus	Not Specified	Trace	Not Specified		Not Specified
embryo d12	E12.0 (c)	TS20: embryo	Not Specified	Trace	Not Specified		Not Specified

Notes:
(a) Corresponding to isoform containing both inserts. Authors state that unequivocal identification of the 72-kDa isoform could not be made.
(b) Corresponding to isoform containing only the 49 amino acid PRR insert.
(c) Age of embryo at noon of plug day not specified in reference.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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