Probe:	gS4
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Schematic illustrations of expression patterns are shown in Figure 12.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Weak	Regionally restricted	5B	Expression is in a zone seprarating the alar and basal plates along the length of the spinal cord and rhombencephalon.
TS15: neural tube	Weak	Regionally restricted	5B	Expression is in a zone seprarating the alar and basal plates along the length of the spinal cord and rhombencephalon.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Weak	Regionally restricted	6B	Expression is in the dorso-lateral wall.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	6D	Expression is as a continuous band along the alar and basal boundary spanning the dorsal thalamus and the pretectum and expression is absent at the isthmus.
TS19: hindbrain	Present	Regionally restricted	6D	Expression is as a continuous band along the alar and basal boundary spanning the dorsal thalamus and the pretectum and expression is absent at the isthmus.
TS19: midbrain	Weak	Regionally restricted	6D	Expression is in the dorso-lateral wall and at the boundary between the dorso-lateral wall and the basal zone in the rostral half and expression is absent in the isthmus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	7B	Expression is as a continuous band along the alar and basal boundary spanning the dorsal thalamus and the pretectum.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	7D	Expression is at the boundary of the dorso-ventral zones.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	7F	Expression is at the boundary of the dorso-ventral zones.
TS19: diencephalon	Present	Regionally restricted	7F	Expression is in a short band located at the alar-basal boundary, from the caudal region of hypothalamus to the rostral region of the ventral thalamus.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	8A	Expression is as a continuous band along the alar and basal boundary spanning the dorsal thalamus and the pretectum.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	8B	Expression is in the caudal region of the hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	8C	Expression is as a continuous band along the alar and basal boundary spanning the dorsal thalamus and the pretectum.
TS20: hindbrain	Present	Regionally restricted	8C	Expression is at the boundary of the dorso-ventral zones.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	8D	Expression is in the caudal region.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	8E	Expression is as a continuous band along the alar and basal boundary spanning the dorsal thalamus and the pretectum.
TS21: thalamus	Present	Regionally restricted	8E	Expression is in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS23: diencephalon	Present	Regionally restricted	8F	Expression is as a continuous band along the alar and basal boundary spanning the dorsal thalamus and the pretectum.
TS23: thalamus	Present	Regionally restricted	8F	Expression is in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	9E	Along the length of the neural tube expression is in the alar and basal plates.

Structure	Level	Pattern	Image	Note
TS20: alar columns	Present	Regionally restricted	9F	Along the length of the neural tube expression is as a bilateral pair of narrow bands at the boundary between the alar and basal plates.
TS20: basal columns	Present	Regionally restricted	9F	Along the length of the neural tube expression is as a bilateral pair of narrow bands at the boundary between the alar and basal plates.
TS20: spinal cord ventricular layer	Present		9F

Structure	Level	Pattern	Image	Note
TS19: midbrain	Weak	Regionally restricted	10A	Expression is in the dorso-lateral wall.
TS19: footplate mesenchyme	Present		10A
TS19: future leg mesenchyme	Present		10A

Structure	Level	Pattern	Image	Note
TS19: footplate mesenchyme	Present		10B/C
TS19: future leg mesenchyme	Present		10B/C

Structure	Level	Pattern	Image	Note
TS21: forelimb digit mesenchyme	Present	Regionally restricted	10D/E	Expression is surrounding the pre-cartilagenous condensations.

Structure	Level	Pattern	Image	Note
TS21: tooth mesenchyme	Present	Regionally restricted	11A	Expression is in the mesenchyme surrounding the enamel organ and include the dental papilla and follicle cells.

Structure	Level	Pattern	Image	Note
TS21: tooth mesenchyme	Present	Regionally restricted	11B/C	Expression is in the mesenchyme surrounding the enamel organ and include the dental papilla and follicle cells.

Structure	Level	Pattern	Image	Note
TS23: tooth mesenchyme	Present	Regionally restricted	11D	Expression is in the mesenchyme surrounding the enamel organ and includes follicle cells.
TS23: dental papilla	Present		11D

Structure	Level	Pattern	Image	Note
TS23: tooth mesenchyme	Present	Regionally restricted	11E/F	Expression is in the mesenchyme surrounding the enamel organ and includes follicle cells.
TS23: dental papilla	Present		11E/F