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Gene Expression Data
Assay Details
Assay
Reference: J:31258 Kozmik Z, et al., Alternative splicing of Pax-8 gene transcripts is developmentally regulated and generates isoforms with different transactivation properties. Mol Cell Biol. 1993 Oct;13(10):6024-35
Assay type: RNase protection
MGI Accession ID: MGI:3038301
Gene symbol: Pax8
Gene name: paired box 8
Probe: Pax8 probe3
Probe preparation: RNA
Visualized with: Autoradiography
Results Image: 8A
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
M Mol. Wt. Marker Lane
E9.5 we E9.5 (a) TS15: conceptus Present (b) 20 µg; total RNA Not Specified Not Specified embryo proper plus extraembryonic membranes plus placenta
E10.5 we E10.5 (a) TS17: conceptus Present (b) 20 µg; total RNA Not Specified Not Specified embryo proper plus extraembryonic membranes plus placenta
E11.5 ep E11.5 (a) TS19: embryo Present (c) 20 µg; total RNA Not Specified Not Specified embryo proper
E12.5 h E12.5 (a) TS20: head Present (c) 20 µg; total RNA Not Specified Not Specified
E12.5 t E12.5 (a) TS20: tail Present (c) 20 µg; total RNA Not Specified Not Specified
E13.5 h E13.5 (a) TS21: head Present (c) 20 µg; total RNA Not Specified Not Specified
E13.5 t E13.5 (a) TS21: tail Present (c) 20 µg; total RNA Not Specified Not Specified
E14.5 h E14.5 (a) TS22: head Present (c) 20 µg; total RNA Not Specified Not Specified
E14.5 t E14.5 (a) TS22: tail Present (c) 20 µg; total RNA Not Specified Not Specified
E15.5 h E15.5 (a) TS23: head Present (c) 20 µg; total RNA Not Specified Not Specified
E15.5 t E15.5 (a) TS23: tail Present (c) 20 µg; total RNA Not Specified Not Specified
E16.5 h E16.5 (a) TS24: head Present (c) 20 µg; total RNA Not Specified Not Specified
E16.5 t E16.5 (a) TS24: tail Present (c) 20 µg; total RNA Not Specified Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) At days 9,5 and 10.5, Pax-8a mRNA is abundantly expressed; levels of variants b and d are considerably reduced; novel splice forms e and f are detected.
(c) Authors state that four mRNA isoforms, a to d, are expressed in the embryo proper from days 11.5 to 16.5, in a ratio which is similar to that seen in the adult kidney.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory