Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:84605 Jeong Y, et al., Distinct regulators of Shh transcription in the floor plate and notochord indicate separate origins for these tissues in the mouse node. Development. 2003 Aug;130(16):3891-902
Assay type:	RNA in situ
MGI Accession ID:	MGI:3037440
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh probe43
Probe preparation:	labelled with digoxigenin RNA

Results

7B ©

Specimen 7B: embryonic day 8.0 (more

)
Note: 5-6 somite stage. Posterior-ventral view.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted	7B	Authors report expression in the node and ventral midline.

7Bi ©

Specimen 7Bi: embryonic day 8.0 (more

)
Note: 5-6 somite stage. Embedded in 4% agarose. Section at the level of the node.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	7Bi	Expression restricted to ventral (mesodermal layer) of node.

7Bii ©

Specimen 7Bii: embryonic day 8.0 (more

)
Note: 5-6 somite stage. Embedded in 4% agarose. Section at level of second most newly formed somite.

Structure	Level	Pattern	Image	Note
TS12: notochordal plate	Present	Regionally restricted	7Bii	Expression restricted to ventral (mesodermal) layer of notochordal plate.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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